这项实验研究对培养过的人眼前房进行灌注。
This laboratory study used perfusion of cultured human anterior segments.
标本被用来检测内毒素水平，灌注后2分钟的标本用来进行培养。
The samples were sent for endotoxin level, as well as samples up to 2 minutes post-perfusion were sent to culture.
方法新鲜脐带内灌注胶原酶消化，获得内皮细胞，用含20%胎牛血清的M199液进行培养。
Methods Endothelial cells were acquired by filling collagen enzyme solution into the lumen of umbilical veins and then cultured in M199 with 20% fetal bovine serum.
新鲜脐带内灌注胶原酶消化，获得内皮细胞，用含20%胎牛血清的M199液进行培养。
Endothelial cells were acquired by filling collagen enzyme solution into the lumen of umbilical veins and then cultured in M199 with 20% fetal bovine serum.
集成的液体贮器使得在需要灌注液体的细胞培养实验期间无需从外部供应液体至培养室。
The integrated reservoirs for liquids remove the need for external supplies of liquid to the culture chamber during cell culture experiments requiring perfusion of liquids.
方法：采用胶原酶灌注法及剪碎消化法分离大鼠胎肝干细胞，并用含10%优等胎牛血清的H -DMEM培养液培养。
Methods Collagenase perfusion method and mechanical cutting method were used to isolate HSCs from rat fetal liver which were then cultivated by H-DMEM containing 10% fetal bovine serum.
方法：采用胶原酶灌注法及剪碎消化法分离大鼠胎肝干细胞，并用含10%优等胎牛血清的H -DMEM培养液培养。
Methods Collagenase perfusion method and mechanical cutting method were used to isolate HSCs from rat fetal liver which were then cultivated by H-DMEM containing 10% fetal bovine serum.
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