目的探讨建立人皮肤汗腺细胞体外分离及纯化培养的技术。
Objective To investigate the method of isolation and purification of epithelial cells of human eccrine sweat gland in vitro.
外泌汗腺细胞CK 7、CK 19免疫组织化学染色均为阳性。
The immunohistochemistry staining of CK7, CK19 was positive in subculture eccrine sweat gland duct cells.
结论:激光照射后,毛囊上皮细胞及大汗腺细胞中有细胞凋亡现象。
Conclusion: Apoptosis exists in hair follicle cells and apocrine gland cells after Laser irradiation.
用质量分数为0 。 %的胰酶和0 .0 %的乙二胺四乙酸 (EDTA)消化分离汗腺细胞和成纤维细胞 ;
The fibroblasts grew along with the growth of eccrine sweat gland cells, and they were separated by digesting with 0 % trypsin and 0 0% ethylenediaminetetraacetic acid (EDTA).
人外泌汗腺细胞的克隆形成率为7.3%,与人胎儿角质形成细胞(7.7%)比较差异无统计学意义(P>0·05)。
There was no obvious difference in the cell clone efficiency between human fetal eccrine sweat gland cells (7.3%) and human fetal keratinocytes (7.7 %, P>0.05).
人外泌汗腺细胞的克隆形成率为7.3%,与人胎儿角质形成细胞(7.7%)比较差异无统计学意义(P>0·05)。
There was no obvious difference in the cell clone efficiency between human fetal eccrine sweat gland cells (7.3%) and human fetal keratinocytes (7.7 %, P>0.05).
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