目的：摸索猪体细胞核移植最佳的融合和激活条件。
Objective: To explore the best conditions of electrical fusion and activation influencing the development of porcine somatic cell nuclear transfer embryo.
第三步是使用一个称为体细胞核移植过程形生一个胚胎。
The third step was to use a process known as Somatic Cell Nuclear Transfer to generate an embryo.
哺乳动物体细胞核移植是一项复杂而有序的操作过程。
Mammalian somatic nuclear transplantation is a complicated and orderful process.
供体核和受体胞质是影响核移植重构胚重编程的主要因素。
The donor cell and recipient cytoplasm are main factors in the nuclear transfer reprogramming.
作者就无透明带核移植技术和单个胚胎培养系统作一介绍。
This paper introduces the methods of zona-free SCNT and the single embryo culture system.
目前，世界上通过体细胞核移植技术已经产生了许多的克隆动物。
So far cloned animals have been produced in many species by somatic nuclear transfer.
外源基因转染后的衰老细胞核移植后能支持克隆胚胎的体外早期发育。
The senescent transfected MEF cells as donors of nuclei could support the early development of cloned embryos after nuclear transfer.
但是核重编程仍是核移植技术的瓶颈，制约了重构胚胎干细胞的研究。
However, nuclear reprogramming, which stands in the way of reconstructed embryo stem cell research, is still a bottleneck in the procedure of nuclear transfer.
本节重点介绍将驱动程序从2.4内核移植到2.6内核的一些重要方面。
This section highlights some of the important aspects of device driver porting from the 2.4 to 2.6 kernel.
细胞工程主要包括体细胞融合，核移植，细胞器摄取和染色体片段的重组等。
The works mainly comprise the body cells, cell fusion, nuclear transplantation, cell uptake and the reorganization of chromosome segment.
猪的核移植研究意义重大，应用价值颇高，是当今生物技术的热点研究之一。
The research of porcine somatic nuclear transfer has huge scientific significance and applied value, which is one of hot point of biotechnology.
体细胞核移植为人们生产不引起免疫排斥以及无内源性病毒的猪提供了可能。
So the current research was designed to study main items of pig somatic nuclear transfer systematically such as somatic donors, recipient oocyte, culture of cloned embryos and embryo transfer.
比较了原代和继代核移植在操作各环节以及核移植胚胎在体外发育能力上的差异。
Bovine cloned embryos were compared with recloned embryos in some aspect of nuclear transfer manipulation and the ability of in vitro development.
将胚胎分割技术与细胞核移植、临床诊断等相结合，将具有更为广阔的应用前景。
There will be wider application prospect if we combine the embryo bisection with new biotechniques such as cell nuclear transfer, diagnosis before child birth, and so on.
治疗性克隆是通过核移植和干细胞技术为病人提供所需更换的细胞、组织乃至器官。
Therapeutic cloning based on application of nuclear transfer and stem cell technology is targeted to provide an unlimited supply of compatible cells, tissue and organs for human transplantation.
逐步成熟的核移植技术，必将给畜牧业和生物学、医学等研究领域带来深远的影响。
This technology would have brought great influences on livestock husbandry, biological, and medical research.
按照供体细胞的不同，哺乳动物核移植研究可分为两种：胚胎细胞核移植和体细胞核移植。
According to the different donor types, mammalian nuclear transfer can be divided into two kinds: embryonic nuclear transfer and somatic nuclear transfer.
“治疗性克隆”是人类最关注的课题之一，而人体细胞核移植是治疗性克隆的基础和前提。
Therapeutic cloning is one of our major research objectives, and therapeutic cloning is based on human somatic cell nuclear transfer.
大鼠是研究人类疾病的理想模型动物，而利用核移植手段是创造遗传修饰大鼠的最佳手段。
The rat is a reference animal model for physiological studies and for the analysis of multigenic human diseases such as hypertension, diabetes and neurological disorders.
细胞核与细胞质的关系是生物学的基本问题，核移植和原核置换是研究核质关系的重要方法。
The relationship between cytoplasm and nucleus is the basic problem of biology, and nuclear transfer (nt) and pronuclear transplantation are the important methods to research their relations.
目的：探讨胞质内直接注核法和电融合法对大鼠-小鼠种间体细胞核移植重组胚发育的影响。
Objective:To study the effect of reconstructed embryos by cytoplasm injection and electrical fusion in rat-mouse inter-species somatic cell nuclear transplantation.
采用SDS-聚丙烯酰胺凝胶电泳及银染法，对兔核移植胚胎蛋白质合成类型的变化进行了研究。
The qualitative patterns of protein synthesis in nuclear transplant rabbit embryos were examined by SDS-polyacrylamide gel electrophoresis followed by silver staining.
核供体和卵母细胞线粒体都损伤的核移植胚发育率的变化规律与卵胞质受体线粒体损伤的核移植胚一致。
After both nuclear donor and ooplasmic recipient mitochondria are damaged, the development pattern of reconstructed embryos is the same with as that of only ooplasmic recipient mitochondrial damaged.
核移植克隆猪技术的出现为生产转基因猪提供了一种有效的方法，并且是目前生产基因打靶猪的惟一方法。
Pig cloning provides an efficient method for producing transgenic pigs, further more, it is the only approach which has been used to produce gene-targeted pigs so far.
本研究在我国首次获得牛细胞核移植的成功，证明用IVM卵母细胞和IVF胚胎进行完全体外化的牛细胞核移植是可行的。
It is the first successful bovine nuclear transfer in China, indicating that it is feasible to transplant bovine nuclear in the whole in vitro procedure using IVM oocytes and IVF embryos.
本研究在我国首次获得牛细胞核移植的成功，证明用IVM卵母细胞和IVF胚胎进行完全体外化的牛细胞核移植是可行的。
It is the first successful bovine nuclear transfer in China, indicating that it is feasible to transplant bovine nuclear in the whole in vitro procedure using IVM oocytes and IVF embryos.
应用推荐