• 检测使用的是世界卫生组织批准的试剂和引物。

    Tests were performed using WHO-approved reagents and primers.

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  • 本研究设计并合成了一对引物。

    A pair of primers was designed and synthesized.

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  • 引物是PCR特异性反应的关键。

    Primer is the key of specific reactions of PCR.

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  • 设计了特异性较好的引物。

    Primers which have high specificity were designed.

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  • 只要把你的模板上加点缓冲液和一些引物。

    Just mix your template with a buffer and some primers.

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  • 缓冲池索引物理读数(以表空间页宽为单位定义)。

    Buffer pool index physical reads (defined in units of tablespace page size).

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  • 每个引物不同的品种能扩增出0 ~ 10个条带。

    For different varieties, 0-10 bands could be amplified with each primer.

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  • 采用不同引物进行扩增时种内变异的程度有所不同。

    By using different primers, the intraspecies variation of RAPD profiles was different.

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  • 这些变化趋势可以作为其它作物引物组合选择的参考。

    This results can be a good reference for other primer selection studies.

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  • 方法设计一对寡核苷酸引物,其上游引物为突变引物。

    Methods A pair of primer was designed and a mismatch nucleotide was introduced in its upstream primer.

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  • 重叠延伸PCR技术成功的关键是重叠互补引物的设计。

    The overlap primers design is the key factor for the successful accomplishment of SOE PCR.

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  • 同时筛选得到14个扩增稳定、多态性丰富的ISSR引物。

    And 14 primers with stable amplification and rich polymorphism for ISSR were screened.

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  • 康德出版了绪论两年后作为引物,希望让他的想法更容易获得。

    Kant published the Prolegomena two years later as a primer, hoping to make his ideas more accessible.

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  • 方法:采用随机引物扩增技术(RAPD)对菌株进行分析。

    Methods: The strains are analyzed using random amplified polymorphic DNA (RAPD).

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  • 根据苯酚羟化酶基因高度保守序列设计一对该基因的特异引物。

    A pair of specific primers of gene encoding phenol hydroxylase was designed by oligonucleotide high conservative sequence.

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  • 目的建立应用任意引物聚合酶链反应进行真菌菌型鉴定的方法。

    Objective To establish the method to identify fungus using the arbitrarily primed polymerase chain reaction (APPCR).

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  • 对其余的15个微卫星设计引物,有10对引物扩增出目的片段。

    Designed the primers of the other 15 new microsatellites, and 10 primers could obtain targeted fragments by PCR amplification.

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  • 标记引物pcr仪相对便宜和快捷,但会忽略那些特殊或罕见的病毒。

    MassTag PCR is relatively cheap and fast, but it can miss viruses that are scarce or especially exotic.

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  • 本文概括介绍了植物不同种属间共用微卫星引物的研究情况及意义。

    The paper deals with the researches and importance of micro-satellite primers shared by different plant species and genera.

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  • 成功进行简并PCR的关键是设计好两组引物库和对反应条件进行优化。

    The optimization of reaction conditions and the primer design are the key factors for the success accomplishment of degenerate PCR.

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  • 用退火控制引物(acp)的差异筛选分析法对该样本进行候选基因的初筛。

    The pooled samples were initially screened for candidate genes for narcolepsy by differential display analysis using annealing control primers (ACP).

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  • 物理地址(处理器用于索引物理ram的地址)的大小限制了可以寻址的内存。

    The size of the physical address (the address used by the processor to index physical RAM) limits the amount of memory that can be addressed.

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  • 方法对测序中模板、引物、测序反应条件及其产物的纯化等因素进行比较研究。

    Methods The effects of DNA templates, primers, cycle sequencing reactions as well as purification methods were comparatively analyzed.

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  • 在有适合引物的条件下,RAPD可以作为判断MRSH医院感染流行的初筛工具。

    RAPD with appropriate primer could be a good screen tool for epidemic surveillance of MRSH infection in hospitals.

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  • 不同引物所扩增出的条带数目片段大小不同,同一条引物在不同晶系之间也有较大差异。

    Three primers were screened and all of them were polymorphic, the band numbers and the fragment length were different in each primer.

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  • 引物设计是RAMP和REMAP标记分析的关键,二者都使用锚定的微卫星序列引物。

    The primer design is a key step for RAMP and REMAP, in which anchored simple sequence repeat primers were used.

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  • 单引物pcr是一种只用一条引物就可以克隆已知序列侧翼未知区域的染色体步移方法。

    Single primer PCR was a method of chromosome walking to isolate sequences flanking a known DNA sequence with only one primer.

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  • 图1抗性基因STV11引物设计策略红色表示引物结合位点,蓝色表示序列差异位点。

    Fig. 1. Design strategies for STV11 marker. Primer binding sites are shown in red, sites with different sequence are shown in blue.

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  • 目的探讨改良扩增前引物延伸(ipep)法对痕量DNA样本s TR检测分型的效果。

    Objective To study the effect of modified improved primer extension preamplification (IPEP) on STR analysis of trace DNA.

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  • 经设计通用引物、PCR扩增、克隆和测序,首次从分子水平鉴定了杂草赛葵上的双生病毒。

    We identified the geminivirus in Malvastrum coromandelianum from the molecular level by designing the primers, PCR, cloning and sequencing.

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