纯化PCR产物，并对其进行序列测定。
PCR产物经限制性内切酶消化、序列测定及分析。
这种着丝粒蛋白的基因克隆、序列测定工作正在进行。
They were sequenced and compared with other isolates from different areas of the world and constructed the phylogenetic tree.
结果序列测定初步确定所克隆的是功能性抗体可变区基因。
Results The cloned antibody variable regions genes were approved functional by sequencing.
基因序列测定和分析结果进一步表明该毒素基因高度保守。
PCR and sequence analysis were used to detect the toxin gene.
由电泳制备的蜗牛木聚糖酶，达到序列测定和制备单克隆抗体的要求。
By the electrophoresis preparation's snail xylanase, achieves the request to determine sequence and to prepare the monoclonal antibody.
由电泳制备的蜗牛木葡聚糖酶，达到序列测定和制备单克隆抗体的要求。
Snail xyloglucanase gained by electrophoresis also can meet the requirements of sequencing and monoclonal antibody.
阳性重组质粒经pcr、酶切鉴定，用双脱氧链末端终止法进行序列测定。
The positive recombinant plasmid was identified by PCR, enzyme digestion. The nucleotide sequence of CSP3 'ending gene was determined by the dideoxy chain termination method.
它们可以被广泛地应用于DNA序列测定、新基因的检测和突变基因的分析。
Gene chips can be widely used for sequencing of DNA, and for detecting new genes and mutations.
研究衍生聚偏二氟乙烯(PVDF)膜用于蛋白质及肽的固相序列测定的可行性。
Objective To investigate the feasibility of derivatized polyvinylidene difluoride (PVDF) membrane used for solid phase sequencing of proteins and peptides.
然后通过对这些基因的拷贝进行序列测定，最终得出肿瘤样本中的基因与正常基因的不同之处。
They then ran those copies through DNA-sequencing machines to see how the samples' genes differed from the reference genes.
目的：对不同粘附力变形链球菌临床分离株表面蛋白V区、P区编码基因进行序列测定。
Objective: To sequence V-region and P-region gene of surface protein of serotype c Streptococcus mutans clinical isolates with different adherent abilities.
目的：从基因组dna获取编码脑源性神经营养因子基因，并对目的基因进行序列测定。
Objective: to amplify the gene for human brain - derived neurotrophin factor (BDNF) directly from human genomic DNA and to assure it by sequencing.
采用RT-PCR方法对口蹄疫病毒O/LZ株基因组序列进行了分子克隆和序列测定。
The genome of Foot-and-Mouth disease virus(FMDV) O/LZ isolate was cloned by RT-PCR, and sequenced.
这项课题采用了DNA序列测定技术并从Markowitz治疗过的肿瘤病人中选择样本。
The project drew on advances in DNA sequencing technology and collections of tumor samples from patients treated by Markowitz.
其中碳末端45 0个核苷酸的序列测定和分析提示，该7株病毒为麻疹野病毒H1基因型。
Sequence analysis of 450bp of C-terminus of the N gene indicated that all of the 7 isolates were H1 genotype.
方法应用PCR及单链构象多态性(SSCP)分析技术结合基因序列测定方法确定突变类型。
Single strand conformation polymorphism (SSCP) essay and sequence analysis of the PCR product were used to ascertain the gene mutation.
采用RT—PCR方法对口蹄疫病毒O/NY00株基因组L片段进行了分子克隆和序列测定。
The L fragment of the genome of foot-and-mouth disease virus(FMDV) O/NY00 isolate was cloned by RT-PCR, and sequenced.
对分离到的HEV71阳性分离株进行VP1编码区基因扩增，核苷酸序列测定和同源进化分析。
And identified HEV71 isolates were performed by gene amplification of VP1 coding region, nucleotide sequencing and homology analysis of evolution.
结果构建的EBO G87和EBO WT重组载体经内切酶双酶切鉴定及核苷酸序列测定证实。
Results The constructed vectors of EBO-WT and EBO-G87 were identified by restriction enzyme digestion and nucleotide sequencing.
通过克隆鉴定和序列测定，获得120个有效片段，其中84个认定为序列表达标签（EST）。
Screening and sequencing present 120 valid sequences, 84 of them belong in expressed sequence tags (ESTs).
方法：通过菌落特征、显微形态、ITS序列测定及其系统发育分析对菌株WC1016进行鉴定。
Methods:The strain of WC1016 was identified by culture characteristics, morphological characteristics, ITS sequence and phylogenetic analysis.
阳性培养物用生化反应、协同凝集试验、代谢抑制试验、聚合酶链反应和DNA序列测定等方法进行鉴定。
Biochemical reaction, coagglutination test, metabolism inhibition test, polymerase chain reaction (PCR) assay, and DNA sequencing were employed to identify the isolated microorganisms.
结果经琼脂糖凝胶电泳和DNA序列测定证实，建立的PCR检测方法具有极高的灵敏度和较好的特异性。
The results of agarose gel electrophoresis and DNA sequence analysis indicated that the PCR method had high specificity and sensitivity.
方法胆红素氧化酶突变体i 402g和C457S通过聚合酶链反应获得，并经氨基酸序列测定加以证实。
Methods The BO mutants I402G and C457S were obtained by site-directed mutagenesis and confirmed by amino acid sequence analysis.
以该融合蛋白为固相抗原，对噬菌体抗体库进行3轮淘筛，并对所获阳性克隆进行抗原结合活性测定和DNA序列测定。
Specific antibody was screened by 3 rounds of panning of phage antibody library with the fusion protein. The antigen binding activity and DNA sequences of positive clones were determined and analyzed.
对BDV阳性产物进行基因序列测定、同源性和氨基酸顺序分析，绘制系统发生树，初探bdv感染的分子流行病学特征。
Further, the gene sequence and amino acid sequence for BDV positive product were analyzed to establish the molecular epidemiologic characteristic by drawing phylogenetic tress.
选择5份标本进行VP1 - 2a基因片段序列测定，测序结果经过比对后发现，5株的VP1 - 2 A序列完全一致。
VP1-2A nucleotide fragments from 5 samples were cloned and sequenced. The results showed that VP1-2A nucleotide fragments of 5 strains were identical.
利用基因组核苷酸全序列测定、电镜观察、血清学测定、传播介体传毒试验等方法对山东省田间发生的玉米病毒病进行了病原鉴定。
Identification on pathogens of maize viral diseases in Shandong province was conducted by whole genomic sequencing, electron microscope observation, serological analysis and vector transmitting test.
利用基因组核苷酸全序列测定、电镜观察、血清学测定、传播介体传毒试验等方法对山东省田间发生的玉米病毒病进行了病原鉴定。
Identification on pathogens of maize viral diseases in Shandong province was conducted by whole genomic sequencing, electron microscope observation, serological analysis and vector transmitting test.
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