目的建立人角膜缘干细胞体外培养方法，观察其生物学特性。
Objective To establish a method for culturing human limbal stem cell in vitro and observe its biological characteristics.
目的建立兔角膜缘干细胞体外培养方法，观察其生物学特性。
Purpose To establish a method for culturing rabbit limbal stem cell in vitro and observe its biological characteristics.
本文旨在探讨人类胚胎来源神经干细胞体外培养的部分生物学特性。
To explored a feasibility source of suitable cell for NSCs-based therapy.
目的探讨人角膜缘干细胞体外培养后细胞形态学、抗原性和增殖能力的变化。
Objective To observe the changes of limbal stem cell morphology? Antigen and proliferative ability after primary culture in vitro.
目的：探讨造血基质细胞对人外周血干细胞体外培养扩增及基因转导的促进作用。
Objective: to inquire into the promoting effect of hematopoietic stromal cells on the culture and ex vivo expansion and the gene transduction of human peripheral blood stem cells.
目的：为保持胚胎干细胞的高度未分化状态，饲养层细胞成为胚胎干细胞体外培养中不可缺少的条件。
AIM: Feeder layer cells are the necessary condition to prevent the differentiation of embryonic stem cells cultured in vitro.
为了探讨人胚神经干细胞体外培养条件和分化情况，摸索出一种切实可行的获得较纯、多潜能人胚神经干细胞的方法。
This is a study on the cultivation condition in vitro and differentiation of neural stem cells from human embryonic brain in order to find a way to get purified multipotential neural stem cells.
建立完善的精原干细胞体外培养体系，为精原干细胞的体外大量扩增提供技术和方法，为治疗男性不育症等提供相关技术。
Finally, to establish the culture system of spermatogonial stem cells in vitro and to offer the therapy technique for the male sterility.
目的探讨人胚神经干细胞体外培养的条件和分化情况，以摸索出一种切实可行的能获得较纯且多潜能人胚神经干细胞的方法。
Objective to study the cultivation condition in vitro and differentiation of neural stem cells from the embryonic human in order to find a way to get purified multipotential neural stem cells.
目的：建立人角膜缘干细胞体外培养模型；探讨单眼角膜缘干细胞缺陷患者自体角膜缘干细胞体外培养后再移植的临床疗效。
Objective To construct the model of human limbal stem cells culture in vitro and evaluate the effect of autologous limbal stem cells transplantation treating with unilateral ocular surface disease.
目的研究人胚胎海马神经干细胞体外长期培养的条件和其在自主分化条件下的分化能力和分化特点。
Objective To study the conditions of in vitro culture and the features of differentiation of neural stem cells from human embryonic hippocampus.
方法小鼠胎脑细胞体外培养获得神经干细胞，并行免疫荧光检测鉴定。
Methods the cells from the embryonic mouse Brian were primary cultured and identified by immunofluorescence stain.
目的： 分离培养睾丸支持细胞、骨髓基质细胞、胚胎成纤维细胞和胰腺成纤维细胞，优化培养条件，建立饲养细胞系，为干细胞体外共培养体系的建立提空充足的饲养细胞。
Objective: Sepretae Sertoli cell, BMSC, MEF, Pancreatic fibroblast and establish cell lines, constructed different feeder layers for a source as a coculture system for stem cells.
目的：比较人胎盘源间充质干细胞和人骨髓源间充质干细胞体外分离培养、扩增及生物学性状的差异。
OBJECTIVE: To compare the differences in isolation and culture in vitro, amplification and differences in biological characteristics between PMSCs and BMSCs.
目的：比较人胎盘源间充质干细胞和人骨髓源间充质干细胞体外分离培养、扩增及生物学性状的差异。
OBJECTIVE: To compare the differences in isolation and culture in vitro, amplification and differences in biological characteristics between PMSCs and BMSCs.
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