方法:以甲三型流感病毒裂解疫苗原液为靶分子,经过3轮生物淘选,从噬菌体随机肽库中筛选与之结合的噬菌体。
Methods: Using the influenza A3 split vaccine as the target molecule, peptides that could bind with target molecule were selected from the phage display 12 peptide library by 3 rounds of biopaning.
同时,本研究所采用的方法也为使用噬菌体随机肽库进行甾体类激素特异性受体(配体)的筛选提供了可靠的实验依据。
The results also show that phage display peptide library is an effective, simple and efficient method to select specific steroid receptors.
并以此为筛选配基,对噬菌体表面展示的随机15肽库进行亲和筛选。
These Abs were used for biopanning of a phage-displayed random15- peptide library.
方法:以高表达人CD59的中国仓鼠卵巢细胞(CHO)为靶细胞,采用竞争结合试验,对噬菌体随机12肽库进行5轮亲和筛选。
METHODS: Using CHO cells which high express human CD59 as target cells, the phage 12 peptide library was screened for 5 rounds by competitive binding test.
目的和方法:用噬菌体随机7肽库对神经胶质瘤母细胞(SWO - 38)进行全细胞筛选,并通过竞争结合实验检验所筛选出的克隆的结合特异性。
AIM and METHODS: The Ph. D. -7 phage display library was used to isolate peptides specific for glioma SWO-38 cell by whole cell screening.
以此纯化蛋白为靶,采用固体包被法对噬菌体展示随机十二肽库及环七肽库进行亲和筛选。
The purified protein coated on polystyrene plate was used as the target to carry out four rounds of bio-panning.
目的应用噬菌体随机七肽库筛选与人CD 137特异结合的肽序列。
Objective to screen polypeptides binding to human CD137 by biopanning phage display random heptapeptide library.
本研究使用纯化的鸡传染性支气管炎病毒(IBV),从12肽噬菌体展示随机肽库中筛选特异性结合多肽。
This study utilized purified IBV H52 strain to screen specific virus-binding peptides from Ph. D. -12TM phage display peptide library.
本研究使用纯化的鸡传染性支气管炎病毒(IBV),从12肽噬菌体展示随机肽库中筛选特异性结合多肽。
This study utilized purified IBV H52 strain to screen specific virus-binding peptides from Ph. D. -12TM phage display peptide library.
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