方法：牛晶体上皮细胞原代培养。
Methods: Bovine lens epithelial cells (BLEC) were cultured in vitro.
结果成功原代培养了猪眼rpe细胞。
Results Primary porcine RPE cells were cultured successfully.
结论：高温可诱发原代培养神经细胞凋亡。
Conclusions: Hyperthermia may induce apoptosis of neuroepithelium in primary culture.
目的探讨体外原代培养大鼠肝细胞的分离方法。
ObjectiveTo explore the isolating methods of rat hepatocytes for primary culture.
目的：探索人脂肪源性基质细胞原代培养方法。
Objective: To establish a primary human adipose-derived stromal cells culture method.
目的：探索原代培养大鼠肝细胞的最佳分离方法。
Aim: To explore the best isolation methods of rat liver cells from primary culture.
方法以无血清培养基体外原代培养角质形成细胞。
Methods Human keratinocytes were primarily cultured in vitro with serum free medium.
本研究拟建立人嗜铬细胞瘤细胞的原代培养方法。
The study is to establish a method of separating and primarily culturing of human pheochromocytoma cells.
目的对大肠腺瘤细胞原代培养的方法作进一步的改进。
Objective To improve the primary cell culture methods of colorectal adenomas.
结论添加晶体皮质可提高晶体上皮细胞原代培养成功率。
ConclusionAdding lens cortex can increase the success rate of primary culture of lens epithelial cells.
迄今为止我们已取得了突变的原代培养的细胞以及蠕虫”。
To date we have made mutant tissue-cultured cells as well as worms.
探讨脑活素对原代培养大鼠大脑皮质神经元的作用及机制。
To investigate the effect of cerebrolysin on primary cultured rat cortical neurons and its mechanisms of action.
说明原代培养细胞在血清中能分化为神经元及神经胶质细胞。
It demonstrated that some of the differentiated cells are neurons and the others were glial cells.
目的探讨淫羊藿甙对体外原代培养神经元缺氧缺糖损伤的作用。
Objective To investigate the effects of Icariin on anoxic and glucose-deprived injury in primary cultured neurons.
目的：探讨温脾汤药物血清对原代培养大鼠系膜细胞增殖的影响。
Objective:To study the effect of Wenpitang pharmacologic serum on proliferation of mesangial cells of rats in vitro.
结论：酶消化法原代培养牛视网膜周细胞是一种稳定、可靠的方法。
Conclusion The enzyme digestion procedure is a stable and reliable method to obtain bovine retinal pericytes.
目的研究原代培养的脊髓神经干细胞在小鼠视网膜的整合和分化情况。
Objective to study the integration and differentiation of spinal neural stem cells after transplantation into mice retina.
方法：膜片钳全细胞记录测量原代培养大鼠海马神经元膜的钾离子电流。
Methods: The potassium currents of rat hippocampal neurons in primary culture were measured with patch clamp whole cell recording.
目的研究氟对原代培养大鼠海马神经元氧化应激和DNA损伤的影响。
Objective To explore the effects of fluoride on oxidative stress and DNA damage in primary hippocampal neurons of rats.
目的：建立一种简单、较理想的新生大鼠皮层神经元体外原代培养方法。
Objective: to establish an easier and better primary culture technique suitable to neurons of newborn rat cortical tis-sure.
研究芳烃类有机溶剂对原代培养大鼠神经胶质细胞的谷氨酸重摄取的影响。
To study effect of aromatic hydrocarbon solvents on the reuptake of amino acid by primary cultured glia cells.
结果：原代培养的RPE细胞含大量色素呈多角形，传代的细胞透明呈梭形。
Results: Primary cultured RPE cells contained large amount of pigments in polygonal shape. Passages of cultured RPE cells presented transparent spindle shape.
本文对23例恶性滋养细胞和19例正常早孕绒毛滋养细胞进行了原代培养。
Primary cell culture was made from 23 malignant trophoblastic tumors and 19 normal early pregnant villi.
兔角膜上皮和内皮细胞组织块原代培养，消化接种于人胚肺饲细胞瓶传代培养。
After primary culture, rabbit corneal epithelium and endothelium were digested and inoculated in bottle having hFLP feeder cells for serial passage.
方法：分别采用组织块法、酶消化法、组织块酶消化法进行人牙髓细胞原代培养。
METHOD: Human pulp cells were primary cultured with tissue block method, enzymolytic method, and tissue block enzymolytic method separately.
为了更为有效地利用该模型小鼠，我们通过原代培养建立了该模型的肿瘤细胞系。
We also established the cell line of this transgenic mice model through primary cell culture to make use this model more efficiently.
目的探讨糖皮质激素对原代培养老鼠脂肪细胞的糖转运活动及胰岛素信号肽的影响。
Objective To explore the effect of glucocorticoid on glucose transport activity and the expression of insulin signaling peptides in the primary cultured rat adipocytes.
研究人员通过建立动物肝脏再生模型和肝细胞原代培养模型，逐渐发现多种效应因子。
By study on animal models of liver regeneration and primary hepatocytes culture, investigators have proved many types of effective factors.
前言：目的：比较不同的人牙髓细胞原代培养方法，优化培养条件，提高培养成功率。
AIM: to compare the primary culture methods of human pulp cells, to enhance the culture condition, and give rise of the success rate of cultivation.
方法：取新西兰白色家兔晶体上皮细胞进行原代培养，细胞融合后用胰蛋白酶进行消化传代。
Methods New Zealand white rabbit lens epithelial cells were primarily Cultured. After the confluence, the cells were trysinized and sub-cultured.
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