采用单链聚合酶链反应(PCR)和测序法检测肿瘤组织中VHL基因的突变情况。
Mutation of VHL gene from tumor tissue was detected from tumor tissue by polymerase chain reaction (PCR) and direct sequencing.
方法：聚合酶链反应（PCR）、单链构象多态性（SSCP）分析。
Methods:Polymerase chain reaction (PCR) and single strand conformation polymorphism(SSCP) was used.
方法采用单管四重聚合酶链反应(PCR)技术。
Methods Single barrel four-seat PCR technique was performed.
目的探讨单管巢式聚合酶链反应(SNPCR)检测石蜡包埋组织结核分支杆菌dna的特异性和敏感性。
Objective To study the sensitivity and specificity of single tube nested polymerase chain reaction (SN PCR) technique in detecting Mycobacterium tuberculosis DNA in paraffin embedded tissues.
用聚合酶链反应和单链构象多态性（ PCR-S SCP）结合序列测定方法，检测了66例健康对照I L-10启动子区多态性的发生。
The Polymorphisms of IL 10 Promoter region were detected by PCR SSCP and sequencing. 66 of health control were examined by this way.
方法：应用免疫组织化学和聚合酶链反应—单链构象多态性分析（PCR-SSCP）检测10例舌白斑和32例舌癌，并结合临床资料进行分析。
Methods:Immunohistochemistry and PCR-SSCP were used to detect the expression of P53 protein and p53 gene mutation in 32 cases of TSCC and 10 cases of tongue leukoplakia.
方法：应用免疫组织化学和聚合酶链反应—单链构象多态性分析（PCR-SSCP）检测10例舌白斑和32例舌癌，并结合临床资料进行分析。
Methods:Immunohistochemistry and PCR-SSCP were used to detect the expression of P53 protein and p53 gene mutation in 32 cases of TSCC and 10 cases of tongue leukoplakia.
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