它具体包括肌干细胞的分离培养和组织工程海绵体的构建。
It specifically includes the isolation culture of muscle stem cells and the construction of tissue engineered corpus spongiosum.
方法体外分离培养人成骨细胞。
Methods The osteoblasts were isolated from human fetal calvaria.
目的分离培养并鉴定人胚胎骨髓间充质干细胞。
Objective To culture and identify mesenchymal stem cells (MSCs) derived from human fetal bone marrow.
方法贴壁法分离培养外周血单核细胞并进行鉴定。
Methods the monocytes were separated by the anchoring technique and identified.
目的从大胚龄人胚脑中分离培养并鉴定神经干细胞。
Objective To isolate and culture human neural stem cells from elder embryonic brain.
目的分离培养人胰岛素瘤细胞，并研究它的功能特性。
Objective To isolate human insulinoma cells, and investigate its functional characteristics.
目的：探讨人表皮干细胞的体外快速分离培养及鉴定方法。
Objective To explore a method for isolation and culture of human epidermal stem cells.
在PSA培养基上，利用稻曲病菌菌核分离培养了稻曲病菌。
Using false smut of rice (FSR) sclerotia isolation, the pathogen of FSR was cultured in PSA medium.
目的分离培养发育基本成熟的人视网膜中神经干细胞样细胞。
Objective To isolate the neural stem cell-like cells from infant human retinas.
前言： 目的：探讨新生大鼠神经干细胞的分离培养和鉴定。
Objective:To study the identification and cultivation of neural stem cells in neonatal rats in vitro.
目的：分离培养兔骨髓基质干细胞并探讨其体外生物学特性。
Objective To study the biological characteristics of rabbit bone marrow mesenchymal stem cells in vitro.
目的建立一种从小鼠骨髓中分离培养间充质干细胞的高效方法。
Objective To establish an efficient method for isolation and culture of mesenchymal stem cells from mouse bone marrow.
方法：制备大鼠急性心肌梗死模型，分离培养同种异体msc。
Methods: Rat model of AMI was performed and allogenic MSC were isolated and cultured.
建立梅花鹿鹿茸软骨细胞的分离培养方法，培养鹿茸软骨细胞。
The methods of isolation and culture sika deer antler chondrocytes were established.
表皮细胞分离培养技术的第一步就是要从皮肤中分离基底层细胞。
Isolation and culture of Keratinocyte, it is the first step to isolate the epidermal basal layer cells.
结论：研究出一套应用少量针刺活检分离培养人MVEC的方法。
CONCLUSIONS We developed a system for isolation and culture of human MVEC from small needle biopsies.
材料：5月龄新西兰大白耳兔用于骨髓间充质干细胞的分离培养。
MATERIALS: New Zealand big-eared rabbits aged 5 months were used for isolation and culture of BMSCs.
目的完善骨骼肌细胞的分离培养方法，为有关实验提供足量的细胞。
Objective To improve the method of isolated culture of mice skeletal muscle cells and supply enough cells for some experiments concerned.
目的提出一个可供分离培养结核杆菌的简易血清琼脂斜面培养方法。
Objective To develop a simple serum-agar slant medium for cultivation and isolation of tubercle bacilli.
目的从新生大鼠的脊髓中分离培养神经干细胞并观察其增殖和分化能力。
ObjectiveTo study the isolation, culture and characterization of neural stem cells from newborn rat spinal cords.
目的改良现有的小胶质细胞纯化分离培养方法，建立稳定简便的培养模型。
Objective To modify the current cultural method of rat microglia and establish a convenient and stable model of culture method for rat microglia.
结论：脐血成分复杂，在体外分离培养可以获得形态各异的脐血间充质样细胞。
Conclusion: Cord blood mesenchymal-like cells can be cultured in vitro, and mesenchymal-like cells with varied morphology can be obtained.
目的：分离培养幽门螺杆菌，为科学研究和药品质量检测提供先进和简便的方法。
Objective: to separate and to cultivate helicobacter pylori, provide advanced and simple method for scientific research and drug control.
但是在乳糖发酵、分离培养和证实试验过程中，有些原理及实验现象需要引起注意。
But some principles and some experimental phenomenons need to be paid attention to during the course of lactose fermentation, separate cultivation and confirmatory test.
目的从成体人牙周组织中分离培养牙周韧带干细胞，研究其分化为软骨细胞的可行性。
Objective to isolate and cultivate human periodontal ligament stem cells (PDLSC) and to investigate the feasibility of PDLSC in vitro differentiation into chondrogenic phenotype.
结论局部应用体外分离培养扩增的单核细胞对神经再生修复有一定的促进作用，其作用优于淋巴细胞。
Conclusion After being purified and amplified in vitro and auto-transferred around the nerve, the monocyte positive effects on facial nerve repair and regeneration are better than lymphocytes.
目的：从泌尿生殖道分泌物中分离培养细菌、支原体及衣原体，了解细菌对多种抗菌药物的敏感情况。
Methods Bacteria, mycoplasma and chlamydia were isolated from the urogenital tract secretion by artifical culture, and their antibiotic susceptibility was detected by disk diffusion.
同时，比较了饲养层细胞类型、生长因子、胚胎发育阶段等因素对绵羊胚胎干细胞分离培养效果的影响。
The effect of feeder cell type, growth factors, and embryo developmental stage on establishment of sheep embryonic stem cell was also studied in present experiment.
同时，比较了饲养层细胞类型、生长因子、胚胎发育阶段等因素对绵羊胚胎干细胞分离培养效果的影响。
The effect of feeder cell type, growth factors, and embryo developmental stage on establishment of sheep embryonic stem cell was also studied in present experiment.
应用推荐