• 母本扩增型相比,共扩增18特异带,表现为多态性

    In comparison with female parents, 18 polymorphic bands are specific bands, namely SSR polymorphism.

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  • 结果扩增出314个条带,其中多态性条带96条,31%。

    Result: 314 bands have emerged, of which 96 are of polymorphism, accounting for 31%.

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  • 叶橐吾三个自然居群总计62个个体使用17个随机引物共扩增出128个多态位点

    Three natural population of l. Sagitta, add up to 62 individual, with 17 random primers, 128 polymorphic loci.

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  • 结果表明,4引物扩增得到272个位点其中269个多位点,总的多态位点比例为98.89%。

    In total, 272 loci were amplified with 4 primer pairs, of which 269 were polymorphic and the percentage of polymorphic loci was 98.89%.

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  • 利用RAPD技术TBT处理细胞提取DNA进行分析,TBT处理组对照组相比扩增出72条差异性片段

    There were 72 differential DNA fragments in the cells disposed to different levels of TBT compared with control by the random amplified polymorphic DNA (RAPD).

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  • 其中88对EST - SSR扩增101个位点并定位到20染色体上(染色体4B没有位点定位)。

    A total of 101 EST-SSR loci amplified from 88 primer sets were distributed onto the 20 chromosomes of the reference maps (no marker on chromosome 4b).

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  • 结果筛选出33个随机引物对68个柞蚕品种(系)扩增出296条DNA带,其中多态性带269条(90.88%)。

    ResultA total of 296 DNA fragments were amplified with 33 primers selected from 68 cultivars of A. pernyi, 269 of which (90.88%) were polymorphic.

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  • 12资源2个莲雾近缘进行了ISSR分析18条引物扩增459个位点其中多态性位点435个(94.77%)。

    ISSR analysis with 18 primers to 12 wax apple(Syzygium samarangense Merr. et Perry)germplasm and 2 closely related species produced 459 unambiguous bands, out of them 435(94.77%) were polymorphic.

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  • 所分析的20个随机扩增出了109条带,但是只有引物s303S4出现4个位点多态性位点所占的比例(P)只有3.7%。

    Among 109 loci revealed by 20 RAPD primers, only primer S303 and S4 showed 4 polymorphic loci. The percent of polymorphic loci (p) was only 3.7%.

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  • 干预40只大鼠不同时间水肿脑组织正常脑组织提取组织rna扩增反转录定量pcr方法测定MMP - 9基因表达水平。

    INTERVENTIONS: Tissues of edema area and normal brain of 40 rats were collected in different time after damage: total RNA were abstracted and MMP-9 gene expression was tested by PCR means.

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  • 结果表明:17扩增154条谱带,其中多态性138条,占89.6%,平均每个扩增出8.12条多态性谱带,表现出丰富多态性。

    There were 138 polymorphic alleles, 89.6% out of 154 amplified loci based on 17 selected ISSR primers with average 8.12 alleles each primer that shown a rich genetic diversity among the population.

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  • 应用23个随机白粉病菌8个不同生理小种进行RAPD分析其中11个引物的扩增型在不同生理小种间出现差异,扩增59条谱带,多态性47.5%。

    Differences were found among 8 strains from RAPD analysis. Out of 23 random primers, 11 were polymorphic. Altogether 59 RAPD bands were found and the polymorphic rate was 47.5%.

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  • 筛选80个RAPD随机物,其中有27个DNA扩增能发现其中抗性基因感性基因区别

    RAPD were selected in this study used 80 random primers, 27 primer pairs of DNA can be amplified and can be found resistance genes and gene perceptual difference.

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  • 应用基因DNA和随机引物,经PCR扩增分离分析,建立了RAPD片段与抗病基因紧密连锁。

    Two PCR amplified fragments were found to be tightly linked to Pi-h-1 (t) by RAPD analysis. of near isogenic pools of DNA from F2 plants.

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  • 在51条随机中筛选出具有稳定扩增条带清晰的多态性引物16条,16条引物检测到132个位点其中多态位点114个(86.36%)。

    RAPD primers were synthesized and 16 primers were selected and used. A total of 132 loci were amplified, of which 114 loci(86.36%) were polymorphic.

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  • 在51条随机中筛选出具有稳定扩增条带清晰的多态性引物16条,16条引物检测到132个位点其中多态位点114个(86.36%)。

    RAPD primers were synthesized and 16 primers were selected and used. A total of 132 loci were amplified, of which 114 loci(86.36%) were polymorphic.

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