克隆文库组成分析、分子杂交和群落指纹图分析被用来分析微生物群落的基因组信息组成。
Clone library profiling, molecular hybridization and community fingerprinting have been used to retrieve genomic information in a community.
建立了筛选质粒表达文库和克隆免疫原基因的技术方法。
We established the techniques of screening the plasmid expression library and cloning the immunogen genes.
它们各自在细菌细胞内复制，产生不同的DNA克隆的“文库”。
These are replicated individually in bacterial cells to produce a library of different DNA clones.
结果证实该文库具有特异性高、假阳性率低、克隆低丰度差异基因高度灵敏的突出优点。
The results demonstrate that the library was high specific , low false positive and sensitive to clone low abundance mRNA.
通过筛选差减文库，共获得了141个克隆。
A total of 141 positive clones were obtained by screening the SSH library.
所构建的消减文库扩增后包含约1000个白色克隆。
用吸附后的血清通过菌落原位免疫杂交筛选该基因组表达文库，从3000个菌落中获得了12个阳性克隆。
The genomic expression library was probed used the adsorbed-pooled sera, and 12 positive clones were obtained from 3000 postulating colonies by immunological hybridization.
随机挑取的100个克隆连续培养5天后的指纹图谱没有发生变化，表明文库中克隆的稳定性。
Randomly picked 100 clones from this library show no fingerprinting changes after 5 days' successive culture, which showed that the clones in the library were stable.
方法采用RD PCR技术构建SHSY5Y细胞基因片段文库，并对每一个克隆进行测序。
Methods The gene fragment library of SH SY5Y cells was constructed with RD PCR technique.
利用寡糖的单克隆抗体，从随机的肽文库中筛选出与之结合力较强的肽序列作为该寡糖的模拟肽。
The peptide's sequences that combine with it tightly can be screened from the peptides' library with the oligosaccharide monoclone antibody.
根据计算机克隆的ZNF322基因序列设计引物，从人类胚胎心脏文库中克隆了ZNF322基因。
The human novel gene of ZNF322 is cloned from human fetal cDNA library using the primers based on the ZNF322 sequence analyzed with computer.
内切葡聚糖酶基因的克隆采取基因文库方法。
Genome library methodology was adopted for cloning of endoglucanase encoding genes.
细菌人工染色体(BAC)是一种承载dna大片段的克隆载体系统，用于人、动物和植物基因组文库构建。
Bacterial artificial chromosome (BAC) is a kind of vector system used to construct large fragment insert libraries of genome DNA in human, animals and plants.
目的构建人噬菌体抗体组合文库，筛选人单克隆抗体。
Objective To construct human phage antibody library and produce human monoclonal antibodies.
目的：消减文库构建过程中，用P CR技术快速筛选重组阳性克隆。
Aim: To develop a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA.
筛选重组阳性克隆可直接用细菌悬液作PCR模板，在消减文库构建时，能大大提高工作效率。
Recombinant clone can be analyzed directly with the use of PCR. It is very efficient, especially in establishment of subtractive library of cDNA.
筛选重组阳性克隆可直接用细菌悬液作PCR模板，在消减文库构建时，能大大提高工作效率。
Recombinant clone can be analyzed directly with the use of PCR. It is very efficient, especially in establishment of subtractive library of cDNA.
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