• On left, red blood cells are stained in wild type zebrafish embryos while on the right mutant embryos lacking the enzyme Ago2 fail to produce blood cells.

    左图,野生型斑马鱼胚胎已染上红色血细胞,而右图缺乏酶Ago2的变异胚胎则不能产生血细胞。

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  • Besides, we also found that the thermal stability of V560A and S561A is much higher than wild type.

    在「热稳定性」的实验中,我发现V560A及S561A这两个突变株对热的抵抗力比野生株来的高。

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  • Transgenic plants were more insensitive to endogenous gibberellic acid (GA) levels than the wild type.

    过量表达植株比野生型对内源赤霉素更不敏感。

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  • But wild type GFP and EGFP are not ideal to detect the transient changes of gene expression regulation.

    但野生型GFP及增强型GFP等因半衰期长,稳定性强,对反映一过性转录表达调控的变化不甚理想。

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  • This mutant showed cooler leaf temperature and faster water loss rate than did the wild type under drought stress.

    在干旱胁迫条件下,该突变体表现为叶面温度低于野生型,且失水率比野生型高。

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  • METHODS: Mouse lymphoma EL4 cells were inoculated subcutaneously into wild type C57BL/6 mice (immune-competent mice).

    方法:给正常野生型C57BL/6小鼠皮下接种小鼠淋巴瘤EL4细胞,建立荷EL4肿瘤的小鼠模型。

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  • The Tm value of the probe hybridized with the wild type PCR products was higher than that of the mutant PCR products.

    检测探针与野生型模板的扩增产物杂交后,其解链温度要高于与突变型产物杂交的解链温度。

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  • Results Compared with wild type mice, there was no significant difference in CBF, CPS and ATP levels in L1 KO mice(P>0.05).

    结果与野生型小鼠相比,L1KO小鼠局部脑血流量、ATP含量和蛋白质合成率差异均无统计学意义(均P>0.05)。

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  • When the DNA was methylated the chromosome was able to be successfully transplanted into a wild type species of M. capricolum.

    当DNA甲基化后,染色体能够顺利植入野生型的丝状支原体。

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  • All three mutants showed higher luciferase activity than that of the wild type, and the V85I mutant showed the highest activity.

    三个突变异种均显示比野生型具有更高的荧光素酶活性,并且V85I突变异种活性最强。

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  • The variant cell line also expressed high level of cross resistance to ethionine which was 6.5 fold higher than that of the wild type.

    同时还表现出对乙硫氨酸的交叉抗性,其抗性是野生型的6.5倍, 表明该抗性系抗性表达稳定。

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  • The crude lipid, crude fiber and apparent amylose contents in the mutant were enhanced significantly compared to those in the wild type.

    突变体的表观直链淀粉、粗脂肪和粗纤维的含量显著提高。

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  • After co-transfection of recombinant plasmid and wild type AcNPV DNA, the rAcNPV was confirmed by the end point dilution assay and dot blot.

    经同源重组后,以终点稀释和斑点杂交法筛选重组杆状病毒;

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  • At the seedling stage, tac2 displayed normal phenotype and its tiller Angle significantly increased at the tillering stage compared with wild type.

    该突变体苗期表型正常,分蘖期株型松散,分蘖角度较野生型显著增大,株高明显降低。

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  • This mutant displays normal phenotype similar to its wild type plants except for the fragility of all plant body, with 10% decrease in the cellulose content.

    与野生型相比,突变体除了整个植株变脆、纤维素含量降低约10%外,表型与野生型相似。

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  • METHODS: The regulating region of the human GCLC gene was cloned by PCR method to construct the wild type plasmid, which expressed luciferase reporting gene.

    方法:通过PCR的方法克隆GCLC基因的部分转录调控区基因,构建表达虫荧光素酶报告基因的质粒;

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  • Objective to construct the expression vectors of human cytochrome P450 (CYP) 4f2 wild type, V81G and V433M Variants and express and purify CYP4F2 protein in e.

    目的构建人细胞色素P450 4f 2基因野生型、V81G和V433 M突变型表达载体,在大肠杆菌中表达并纯化出CYP4F2蛋白。

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  • We calculated the rosette leaf number at different developmental stages, and found that the number of rosette leaves of mutant did not differ significantly from wild type.

    统计了不同发育时期的莲座叶叶片数目,发现突变体与野生型的莲座叶叶片数目没有差异。

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  • Moreover, pre-treatment of wild type mice with a nitric oxide (. NO) synthase inhibitor prevented the HBO2-induced elevation in stem cell factor and circulating stem cells.

    而且,野生型小鼠用NO合酶抑制剂预处理后阻止了HBO2介导的干细胞因子和外周血干细胞的增加。

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  • Importantly, transgenic plants accumulated higher fresh weight under long-term osmotic stress, and also have shown retention of more water than the wild type during drought stress.

    更重要的是,与野生型相比,转基因植株在长期的渗透胁迫下会积累更高的鲜重,同时在干旱胁迫中能够保留更多的水分。

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  • RESULTS: DDR2/EGFP fusion plasmid was successfully constructed. Further analysis also demonstrated that the fusion protein has similar expression and activation pattern with wild type ones.

    结果:成功构建了DDR2/EGFP融合表达载体,进一步的分析也证明此融合表达载体能在细胞中正确表达并可以被配体所激活。

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  • Besides, the strain is wild type with very clear genetic background, which is fit for genetic improvement and is hopeful to improve the capability of disposing the organic waste gas greatly.

    此外,该菌株为野生型,遗传背景非常清晰,适于进行遗传改良,有望大幅度提高对有机废气的处理能力。

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  • They all accumulated on the surface of rice root system in the medium with higher ammonia concentration, while the wild type strains were removed from rice root surface in the same condition.

    这些转化结合子在高铵下表现出固氮活性,并能集聚在水稻根表,而野生型菌在相同条件下远离水稻根表。

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  • Conclusion Construction of the eukaryotic expression vectors of wild type and mutant PRPF31 genes is basic work for research on the mechanisms of retinitis pigmentosa caused by PRPF31 mutation.

    结论野生型和突变型PRPF31基因真核表达载体的构建,为研究PRPF31基因突变引起视网膜色素变性的机制奠定了基础。

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  • Objective: the wild type FHIT gene was transfected into the human gastric cancer cell line MGC-803 in which the FHIT gene had been totally lost so as to investigate its effect on Adriamycin (ADM).

    目的:将FHIT基因导入该基因表达缺失的人胃癌细胞株mgc- 803,探讨胃癌中FHIT基因表达对阿霉素(adm)敏感性的影响。

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  • MDA contents of both transgenic and wild type plants increased under the drought and high-salt stresses, but MDA contents in wild type plants increased much more than that in the transgenic plants.

    干旱和盐胁迫导致野生型和转基因型植株丙二醛含量升高,但野生型植株的丙二醛含量增加量要远远高于转基因植株;

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  • We found that 9 out of 24 (38%) patients whose tumours were initially KRAS wild type developed detectable mutations in KRAS in their sera, three of which developed multiple different KRAS mutations.

    结果发现,在24名治疗前KRAS为野生型的患者中有9名(38%)患者的血清检测出突变型KRAS。其中3名的突变为多位点的不同突变。

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  • The authors found that mice lacking CD24, a membrane protein expressed by immune and stem cells, developed an inflammatory response that was more powerful and lethal than that in wild-type mice.

    作者们发现,缺乏CD 24,这种在免疫细胞和干细胞上表达的膜蛋白的老鼠,产生了比野生型老鼠更强且更致命的炎症反应。

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  • Complete response rates were similar for children with either mutation and children with wild-type FLT3, the authors report.

    作者报道,有突变患儿和野生型FLT3患儿的完全有效率是相似的。

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  • Clinical and pathological variables, copy number alterations, mutations, and expression signatures were compared with a cohort of TCEB1 wild-type tumors.

    就肿瘤的临床及病理特征、染色体拷贝数量改变、突变和表达特征等方面与TCEB1野生型肿瘤进行比较。

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