Objective To construct the clone vector of S gene in occult hepatitis B virus infection. To analyse its mutations and to construct its prokaryotic expression vector.
目的构建克隆载体，分析隐匿性乙型肝炎病毒S基因的突变情况，构建其原核融合蛋白表达载体。
The influence of each vector on the cell proliferation, clone-formation and alteration of cell cycle of bladder cancer cell line EJ were observed in vitro.
分别转染膀胱癌细胞株EJ ，观察不同载体对膀胱癌细胞体外增殖、克隆形成和细胞周期时相的影响。
Objective to clone human heat shock protein 70 (HSP70) gene for the construction of a prokaryotic expression vector.
目的克隆人热休克蛋白70 (HSP70)基因，构建其原核高效表达载体。
Objective To construct the recombinant plasmid that highly expressed human zona pellucida 3. Methods The techniques of PCR amplification, T-A vector ligation, and sub-clone were used.
目的：构建高效表达人透明带蛋白3的真核重组表达载体。方法：利用PCR、T- A载体克隆和亚克隆等技术。
Objective To construct the recombinant plasmid that highly expressed human zona pellucida 3. Methods The techniques of PCR amplification, T-A vector ligation, and sub-clone were used.
目的：构建高效表达人透明带蛋白3的真核重组表达载体。方法：利用PCR、T- A载体克隆和亚克隆等技术。
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