Our method extended the utility of universal primers and provided implications for primer design and PCR amplification of some other genes.
新方法扩展了“通用引物”的适用范围,并为引物设计和一些其它基因的PCR放大提供了思路。
Mitochondrial cytochrome oxidase subunit I (COI) fragments of Sparus macrocephalus and Sparus latus were amplified using universal primers.
利用通用引物成功扩增了黄鳍鲷和黑鲷的线粒体细胞色素氧化酶i亚基(COI)基因序列。
Objective: To set up a rapid and simple PCR assay for Candida detection and identification by using internal tran scribed spacer (ITS) universal primers.
目的:应用内转录间隔区(its)通用引物建立一种较为快速、简便的检测和鉴定念珠菌的聚合酶链反应(PCR)方法。
Methods We used a hot initiated PCR based method with asset of universal acanthamoeba specific primers to detect acanthamoeba.
方法以一段特异性通用引物并配合热启动聚合酶链反应技术来检测临床标本中的棘阿米巴原虫。
Methods We used a hot initiated PCR based method with asset of universal acanthamoeba specific primers to detect acanthamoeba.
方法以一段特异性通用引物并配合热启动聚合酶链反应技术来检测临床标本中的棘阿米巴原虫。
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