Objective To construct a bait plasmid in bacterial two-hybrid system.
目的构建大肠杆菌双杂交系统的诱饵重组质粒。
Conclusion:The bait vector FOXL2 in yeast two-hybrid system is constructed successfully.
结论：成功构建了正常及突变型的FOXL2酵母杂交诱饵质粒；
Bacterial two-hybrid system is a newly developed method for studying protein-protein interaction.
细菌双杂交系统是新近建立的一种研究蛋白质间相互作用的方法。
The dimerization domain of NEMO was mapped to residues 251-419 by deletion and yeast two-hybrid analyses.
我们首先应用缺失突变和酵母双杂交试验将NEMO的二聚化域确定于251-419残基。
These lay a rigid basis for investigating the proteins interacting with FOXL2 by yeast two-hybrid technique.
为进一步利用酵母双杂交技术研究与FOXL2相互作用的蛋白打下坚实基础。
The yeast two-hybrid system is a novel and sensitive technique to detect protein-protein interactions in vivo.
酵母双杂合系统是在酵母细胞中研究蛋白- 蛋白相互作用的新技术。
During the procedure, different molecular biology techniques were involved such as PCR RT-PCR and Yeast-two-hybrid system.
在试验过程中用到了很多高级的生物学技术，比如说PCR,RT - PCR和酵母双杂交技术。
Conclusion Using yeast two-hybrid system, we got some alternative proteins which may interact with the cytoplasmic tail of NECL1.
结论应用酵母双杂交系统，获得了一些候选的NECL1胞内区相互作用蛋白。
CONCLUSION: Yeast two-hybrid GAL4 system 3 can be utilized to fish HCV core region-interacting protein. HCV core protein does not have self-activating function.
结论利用酵母双杂交Gal4系统3研究与HCV核心蛋白相互作用的蛋白，证实了HCV核心蛋白无自激活功能。
In order to study the functions and downstream signal transduction mechanisms of these two genes, yeast two-hybrid analysis of the two genes was made in this paper.
为进一步研究这两个基因的功能及下游信号传导机制，本试验对这两个基因作了酵母双杂交分析。
The endocrine disrupting functions of 4 natural and synthetical estrogens, 4 phytoestrogens, 14 phenols widely used in the tap water supply system were detected using yeast two-hybrid technique.
利用酵母双杂交系统对4种天然和合成的雌激素，4种植物性激素以及14种常用于自来水供水系统中的酚类物质进行了内分泌干扰作用的筛检测定。
Some new type of two-hybrid systems, such as split-ubiquitin system, protein-fragment complementation assay, repressor reconstitution assay and SOS recruitment system, have been developed recently.
前言： 近年来，一些不依赖于转录因子活性的新型双杂交系统相继建立，如分离的泛素系统、 蛋白质片段互补分析、阻遏物重构分析和SOS恢复系统等。
The yeast two-hybrid analysis demonstrated that CK1A and CRY2 can interact in vivo under blue light, which indicates that CK1A may play an important role in blue light signal induction of Arabidopsis.
酵母双杂交结果显示CK1A蛋白在蓝光下能与CRY2蛋白发生相互作用， 暗示CK1A基因可能参与拟南芥的蓝光信号传导途径。
"I have two dreams. One is to enjoy the cool under the tall rice, and the other is to grow hybrid rice across the world," Yuan said.
“我有两个梦想。一个是享受高大的水稻下的凉爽，另一个是让全世界都种上杂交水稻。”袁说。
Yuan once said he had two dreams—to "enjoy the cool under the rice crops taller than men" and that hybrid rice could be grown all over the world to help solve the global food problem.
袁曾经说过他有两个梦想——“在比人高的水稻下乘凉”，以及杂交水稻可以在世界各地种植，帮助解决全球粮食问题。
And some applications will be a hybrid of those two approaches.
并且，有些应用程序将是这两种方式的混合。
In the following two articles, you will see how the hybrid model, binding a local application with cloud services, is achieved.
在接下来的两篇文章中，我们将了解绑定本地应用程序与云服务的这个混合模型是如何实现的。
Two increasingly popular forms of social enterprises are hybrid nonprofits and social businesses.
两种日益流行的社会企业模式是混合型非营利组织和社会商业。
When they analyzed DNA to determine its relationship to others, they were stunned to find that it was a hybrid of two closely related flies, the blueberry maggot and the snowberry maggot.
研究人员对忍冬蝇的DNA进行分析，以确定它们和其他果蝇之间的亲缘关系，结果相当令人惊讶，忍冬蝇竟然是蓝莓蝇和雪莓蝇这两种关联果蝇物种的杂交后代。
In a true hybrid, the genetic material from the two species is mingled at conception, with half the chromosomes coming from one parent and half from the other.
在实际的融合过程中，由两个物种的遗传物质在概念上是可交融的，染色体的一半来自父母一方，一半来自另一方。
All hybrids have two fuel tanks; one holds gasoline and the other electrons (the hybrid battery pack).
所有的混合动力车有两个箱子：一个装汽油而另一个是电池。
If we think about bringing in those last two carbons, what you can see is that for every carbon, two of its hybrid orbitals are being used to bond to other carbons.
如果我们考虑引入最后两个碳原子，你会看到的是对于每个碳原子，其中的两个杂化轨道，和另外的碳原子成键。
The two strains whose genes are found in the hybrid belong to influenza families known generally as North American and Eurasian pig flu.
在加利福尼亚病毒样本中发现的两种菌株的基因很常见，属于北美和欧亚猪流感株。
The two-passenger hybrid aims to be fuel efficient, easy to repair, safe to drive and inexpensive to own.
这辆双座位的混合动力汽车的目标是省油，容易维修，驾驶安全，并且购买价格低廉。
Along with BMW, General Motors and Chrysler, it has developed a new hybrid system called Two-Mode.
除了BMW，通用汽车和克莱斯勒也开发了名为“双模式”的混合动力系统。
Honda, the second-largest Japanese carmaker, announced plans to launch two petrol-electric hybrid cars next year.
日本第二大汽车制造商本田汽车计划明年生产两台汽油电力混合动力车。
Is DTC genetics medicine, recreation, or some strange hybrid of the two?
还有其它DT C基因药物、基因再造以及这两者奇怪的结合产物又是怎样的呢？
According to Baumann, A. tesselata is lucky: it appears to be descended from a union of two related species, giving it a hybrid vigor.
而鲍曼表示，a .tesselata是很幸运的：这种生物是两种有亲缘关系的物种的杂交后代，所以它有杂交优势。
So again, looking at the shapes, now we're just combining two, we've got these two equal hybrid orbitals plus these 2 p orbitals here.
同样，我们看它的形状，现在我们仅仅结合两个轨道，我们得到这两个杂化轨道,和两个p轨道。
So again, looking at the shapes, now we're just combining two, we've got these two equal hybrid orbitals plus these 2 p orbitals here.
同样，我们看它的形状，现在我们仅仅结合两个轨道，我们得到这两个杂化轨道,和两个p轨道。
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