Human CD3AK cells were transfected by viral supernatant.
用病毒上清感染人CD3AK细胞。
Retrovirus vector; Stable transfected cell line; Stem cells.
逆转录病毒；稳定转染；干细胞。
Then, the content of melanin in antisense TRP-1 transfected melanocyte was detected.
测定TRP - 1反义核酸转染黑素细胞后细胞内黑素含量的变化。
These recombinant plasmids were transfected into PK-15 cells and were screened by G418.
重组干扰载体经纯化后转染PK-15细胞，并进行G418抗性筛选。
The capacity of invasion and metastasis of the transfected cells significantly decreased.
细胞侵袭转移能力明显下降。
Then the recombinant plasmid was transfected into NK-92 cells with Hpofectamine encapsuled.
然后，通过脂质体转染法将其转入NK-92细胞；
We also showed that the IRF-7 was localized mainly in the cytoplasm of transfected 293t cells.
使用该抗体证实，转染293t细胞后表达的IRF - 7主要分布在细胞质内。
DKK1 antibody detects transfected levels of DKK1 protein. This antibody does not cross-react with DKK2.
DKK1抗体识别转染水平的人源dkk1蛋白，此抗体与DKK2没有交叉反应。
Objective to optimize the selective method to obtain the pure recombinant plasmid transfected U937 cells.
目的：探索优化重组质粒转染细胞后的筛选方法，以得到高纯度U 937转染细胞。
The intracellular antibody genes were transfected into the human pulmonary giant cell carcinoma PG cells.
对人巨细胞肺癌PG细胞系进行基因转染。
The transfected cells were inoculated into nude mice, and the tumor growth inhibition rate was calculated.
体外转染乳腺癌细胞，常规动物接种，测量肿瘤体积和动物重量，计算肿瘤抑制率。
The plasmids in cDNA library and in gene pools were extracted and NIH/3T3 cells were transfected respectively.
将文库菌落印迹至尼龙膜，分区培养提取质粒DNA ，建立基因池，并分别转染NIH/3T3细胞。
Objective To inject RF B cell transfected by lentivirus to arthritis rats, and to evaluate the curative effect.
目的慢病毒转染的RFB细胞静脉注入类风湿性关节炎大鼠，评价其治疗效果。
To assay the transfected cells by immunohistochemical and insulin levels were monitored by radioimmunoassay( RIA).
并用放射免疫分析法检测细胞培养液胰岛素水平。
The characteristic DNA ladder was found by the agarose gel electrophoresis for the nucleus DNA of transfected cells.
细胞核DNA琼脂糖凝胶电泳显示凋亡细胞特有的梯状条带。
The recombinant vector was transfected into HEK293 cells with liposome transfection reagents for transient expression.
脂质体法转染HEK293细胞，用倒置荧光显微镜检测、分析其在HEK293细胞表达及定位。
The membrane surface antigens of DNA-transfected cells were detected with cellular ELISA and sandwich ELISA respectively.
分别用细胞和夹心酶联免疫吸附试验（ELISA）检测DNA转染细胞膜表面抗原。
The antisera can recognize not only recombinant IRF-7 expressed in E. coli, but also transfected IRF-7 in mammalian cells.
该抗血清不但能识别来源于大肠杆菌的抗原，还能检测真核细胞内转染后表达的IRF-7。
Methods: MKP 1 gene was transfected into vascular fibroblasts by the classical calcium phosphate coprecipitation technique.
方法：用磷酸钙共沉淀的方法向血管成纤维细胞转染MKP1的基因；
Results rat IL-10 eukaryotic expression vector had been constructed successfully, and had been transfected into rat chondrocyte.
目的构建大鼠il - 10真核表达载体并在大鼠软骨细胞中进行表达。
Objective To study the reverse effect on the malignant phenotype of human NPC cells CNE2 transfected with EBV-LMP1 antisense gene.
目的研究EB病毒LMP1反义基因对人鼻咽癌CNE2细胞恶性表型的逆转作用。
The senescent transfected MEF cells as donors of nuclei could support the early development of cloned embryos after nuclear transfer.
外源基因转染后的衰老细胞核移植后能支持克隆胚胎的体外早期发育。
AIM: To investigate the effects of transfected JWA ribozyme gene on phenotype and migration of human pulmonary artery smooth muscle cells (PASMCs).
目的：探讨JWA核酶基因转染对肺动脉平滑肌细胞表型及迁移的影响。
The Adriamycin-induced DNA damage was compared between the transfected and the untransfected cells by sing le cell gel electrophoresis assay(SCGE).
单细胞凝胶电泳（SCGE）比较阿霉素诱导的转染前后细胞DNA损伤修复情况。
Objective To study the method of immunotherapy against t cell lymphoma by tumor cells transfected by GM-CSF gene combined with interleukin 12 (IL-12).
目的探讨用转粒细胞巨噬细胞集落刺激因子(GM CSF)基因瘤苗，联合白介素12 (IL 12)治疗小鼠t细胞淋巴瘤的方法。
Objective To study the tumorigenicity and metastasis of poorly immunogenic murine B16 melanoma cells transfected with murine interleukin12 (IL12) gene.
目的探讨白细胞介素12（IL12）基因转移对弱免疫原性的小鼠B16黑色素瘤细胞生长与转移的影响。
Objective Antisense CDK4 was transfected into human colorectal cancer cells HT29 to examine the effect of it on the growth and proliferation of HT29 cells.
目的将反义CDK4导入人结肠癌细胞株ht29细胞，观察其对肿瘤细胞生长的影响。
Objective to study antitumor effect of calcium folinate (CF) and 5 fluorocytosine (5 FU) on rectal carcinoma cells transfected with cytosine deaminase (CD) gene.
目的：探讨亚叶酸钙(CF)联合5氟胞嘧啶(5FU)对转染胞嘧啶脱氨酶(CD)基因的直肠癌细胞的杀伤作用。
Objective to investigate the epithelial growth factor (EGF) expression of EGF gene-transfected keratinocytes and its effect on cell proliferation after grafting.
目的探讨转染表皮细胞生长因子(EGF)基因的人表皮细胞移植后egf的表达及对细胞增殖的影响。
Objective to investigate the epithelial growth factor (EGF) expression of EGF gene-transfected keratinocytes and its effect on cell proliferation after grafting.
目的探讨转染表皮细胞生长因子(EGF)基因的人表皮细胞移植后egf的表达及对细胞增殖的影响。
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