Mammalian cell lines from different genus and different tissues can be infected by Bac-GFP, but their transduced cell rate is extremely different.
GFP可感染不同种属不同组织的哺乳动物细胞系,但转导率有差异。
Objective to study the influence on tumorigenicity of human renal cell carcinoma (RCC) cells transduced with human IL 2 gene, and to intend in forming a basis for RCC gene therapy.
目的研究转人IL2基因对原代肾细胞癌(RCC)细胞致瘤性的影响,为RCC的基因治疗提供依据。
Methods a retroviral vector containing TK gene was constructed and transduced into pulmonary adenocarcinoma cell A549 by electroporation.
方法构建含tK基因的逆转录病毒表达载体,电穿孔法转化肺癌细胞A549。
Methods a retroviral vector containing TK gene was constructed and transduced into pulmonary adenocarcinoma cell A549 by electroporation.
方法构建含tK基因的逆转录病毒表达载体,电穿孔法转化肺癌细胞A549。
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