To establish a TaqMan-based real-time fluorescent quantitative PCR assay for detection and quantitation of Hepatitis E virus.
目的建立灵敏、稳定、特异的实时荧光PCR方法，用于戊型肝炎病毒的定量检测。
Objective:To set up a real-time fluorescent quantitative PCR assay for rapid detection of norovirus(NV) RNA in acute gastroenteritis.
目的：建立诺如病毒的荧光定量PCR检测方法，应用于急性胃肠炎的快速检测。
Article on the principle of real-time fluorescent quantitative PCR, factors and plant diseases and genetic breeding of the application were reviewed.
文章对实时荧光定量pcr的原理、影响因素以及在植物病害和遗传育种方面的应用等进行了综述。
Methods:Viral RNA Was extracted from feces. 92 samples were detected by real-time fluorescent quantitative PCR and Enzyme-linked Immunosorbent Assay(ELISA).
方法：提取粪便中的RNA，用实时荧光PCR方法对丽水市急性胃肠炎的92份标本进行检测，并与常规ELISA检测结果比较。
Real-time fluorescent quantitative PCR is a kind of technique that can quantify the nucleic acid on different fluorescence, as it features high sensitivity, accuracy, and specificity.
实时荧光定量PCR技术是一种利用荧光检测方法来定量核酸的技术，具有高度的灵敏性、特异性和精确性。
Results:Norovirus was successfully detected from epidemic sample of Lishui, the positive rates of real-time fluorescent quantitative PCR and ELISA were 78.26% and 55.43%, respectively.
结果：用实时荧光PCR方法成功的从丽水疫情样本中检测出诺如病毒，实时荧光PCR和ELISA的检出率分别为78.26%和55.43%。
Scientists in the us, Japan and in Europe previously have cloned fluorescent mice and pigs, but this would be the first time dogs with modified genes have been cloned successfully, Lee said.
李炳春称，美国、日本和欧洲的科学家此前已克隆出荧光鼠和荧光猪，但成功克隆出转基因狗在世界尚属首例。
Early iterations of the compact fluorescent light bulb were expensive, and consumers complained of myriad problems - from buzzing, flickering and long start-up time to unpleasant hues.
早期的复合型荧光灯管是昂贵的，消费者抱怨各种各样的问题诸如从有响声，闪烁，过长的启动时间产生令人不愉快的色彩。
To forecast the life of vacuum fluorescent display (VFD) in shorter time, an experimental scheme of the accelerated predicted-life test was presented.
为了能在较短的时间内预测真空荧光显示屏(VFD)寿命，提出了一种加速寿命预测的试验方案。
By using lanthanide ions, especially europium ions as the fluorescent labels of samples, TFFBS can perform time resolved fluorescence detection from spectral characteristics of europium ions.
该传感器以镧系离子(铕离子)作为被检测物质的荧光标记物，根据铕离子的荧光特性，利用时间分辨检测技术进行生物医学检测。
To develop a fluorescent MGB probe real time PCR platform for detection the mutation of phenylalanine hydroxylase gene in patients with phenylketonuria.
探讨荧光mgb探针实时PCR技术检测经典型苯丙酮尿症的基因突变。
The decay of light strength and relations between fluorescent powder concentration and film thickness, and light strength and light afterglow time were discus…
讨论了荧光粉含量、涂层厚度与荧光光强、余辉时间等的关系。
The PAM ZERO can directly measure (with no need for fluorescent tagging) a variety of aggregation phenomena, plotting reaction curves in real time without any complex sample prep.
PAMZERO可以直接测量各种聚集现象(不需要荧光标记)，绘制实时反应曲线，无需复杂的样品制备。
The data of the time resolved fluorescent spectra and fluorescent life time of an organic optical disk named the azo dye doped thin film are given.
实际测量了一种有机光盘存储记录材料偶氮染料掺杂薄膜的时间分辨荧光光谱和荧光寿命。
Therefore, the non-fluorescent agent of poisonous chemical substances, is the health and quarantine departments in Baoji City approved a one-time green paper cups.
因此，不含荧光剂等对人体有害的化学物质，是经宝鸡市卫生检疫部门认可的一次性环保纸杯。
Finally, the translocation of fluorescent HBVin living cells was observed by time-lapse confocal microscopy.
最终，应用激光共聚焦显微镜在活细胞中直接观察荧光hbv的转运。
Methods: The incubation time was 2 hours, 12 hours and 24 hours, respectively. Four fluorescent probes were used to label four subcellular organelles.
方法：孵育时间分别为2小时、12小时、2 4小时，四种荧光探针标记四种细胞器。
Methods:The incubation time is 2 hours and 24 hours, respectively. Four fluorescent probes are used to label four subcellular organelles.
方法 ：孵育时间分别为2小时、2 4小时 ，四种荧光探针标记四种细胞器。
The best time for fluorescent screening was the 5th to 8th day of embryo.
荧光筛选的最佳时期在胚胎发育第5到第8天；
Sothenext time you long fortheromance ofthenorthern lights switch on a fluorescent lamp!
所以下次你渴望看到浪漫的北极光时，点亮一盏日光灯吧！
Sothenext time you long fortheromance ofthenorthern lights switch on a fluorescent lamp!
所以下次你渴望看到浪漫的北极光时，点亮一盏日光灯吧！
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