Methods Current-clamp recordings with the use of the whole cell configuration of the patch-clamp technique were made from the primary cultured spiral ganglion neurons.
方法应用膜片钳技术全细胞构型从原代培养的螺旋神经元获得电流钳记录。
The patch clamp technique bring about new light set in studying on mechanism of atherosclerotic development.
膜片钳技术给动脉粥样硬化发病机理研究带来了新的亮点。
METHODS The patch clamp whole cell recording technique was used.
方法采用膜片钳全细胞式记录技术。
The widely using of patch clamp technique has greatly promoted our knowledge of ion channels.
膜片钳技术的广泛应用极大推动了人们对离子通道的认识。
Because the differences of its application, the microelectrode can be divided into ion-selective microelectrode and patch clamp detecting technique.
根据检测目的的不同，微电极主要应用可以分为离子选择微电极检测技术和膜片钳技术。
CONCLUSION: the patch clamp technique makes it possible to observe the action sites of nicotine receptor antagonists.
结论：膜片钳技术使探讨烟碱受体拮抗药的作用位点成为可能。
The first step of recording calcium channel current of cardiomyocytes with patch clamp technique is dissociation of myocytes.
用膜片箝技术记录心肌细胞钙通道电流，首先必须获得单个心肌细胞。
Mechanical dissociation was used to obtain single primary spermatocytes from guinea pigs and iron channel currents in the primary spermatocytes were recorded by whole cell patch-clamp technique.
笔者应用机械分离获得豚鼠单个初级精母细胞，采用全细胞式膜片钳技术记录该细胞离子通道电流。
Using the whole-cell patch-clamp technique, clear evidence was provided for the existence of a hitherto unreported, voltage-dependent nonselective cation current (NSCC) in rabbit ventricular myocytes.
应用全细胞膜片钳制技术，我们首次报道了在兔的心室肌细胞存在一种电压依赖性的非特异性阳离子流（NSCC）。
The patch clamp recording techniques is an advanced electrophysiological technique, which has been widely used for research in the life science research field.
膜片钳技术是一种先进的电生理技术，在生命科学研究中已得到了广泛的应用。
The whole-cell patch-clamp recording technique was used to record the change of sodium channel current influenced by carvedilol.
方法用急性酶解法获得单个大鼠心室肌细胞，标准的全细胞膜片钳技术记录钠通道电流。
Methods: The isolated neurons of dorsal root ganglion (DRG) were studied using the whole cell patch clamp technique in current clamp mode.
方法：应用全细胞膜片箝电流箝制技术，对急性分离的大鼠DRG细胞进行电流箝制。
Methods Cell-attached mode of patch clamp technique were employed to record the single channel current of NMDA receptor with the primary-cultured hippocampal neurons from rats as samples.
以原代培养的大鼠海马神经元为标本，运用膜片钳技术的细胞吸附式方法记录NMDA通道的单通道电流。
Whole-cell patch clamp technique was used to study the electrophysiological characteristics of nociceptive trigeminal ganglion (TG) neurons labeled by nuclear yellow (NY).
实验利用全细胞膜片钳技术研究了荧光染料核黄（NY）标记的支配牙髓的痛感觉神经元的电生理特征。
Method The experiment was carried out in freshly isolated bullfrog using a whole-cell patch-clamp technique.
方法实验采用的方法是全细胞膜片钳技术，实验材料为急性分离的牛蛙背根神经节神经元。
The characterizations of voltage-activated potassium channels were studied on newborn bovine retinal neurons in long-term cultures with whole-cell patch clamp technique.
目的探讨体外培养不同时期新生小牛视网膜神经元电压门控钾离子通道的特性。
METHODS: The date recording from cell - attached patches of patch - clamp technique.
方法：采用细胞贴附式的膜片钳技术。
The effects of streptomycin on ionic currents in outer hair cells isolated from the cochlea of guinea pig were investigated using a whole-cell recording patch-clamp technique.
使用全细胞式记录膜片钳技术，研究链霉素对豚鼠耳蜗外毛细胞离子电流的影响。
The sodium currents were detected by patch-clamp technique in whole-cell recording mode.
用膜片钳技术的全细胞模式测定全细胞钠电流并观察应用特布他林对它的影响。
This article reviewed lately research status of Chinese herbal effect on calcium channels of myocardial cell by patch clamp technique and look ahead the future research.
本文综述了近年来利用膜片钳技术研究中药对心肌细胞膜钙通道作用的现状，并对未来研究进行展望。
Method: the whole cell patch clamp recording technique was used in this study. Results: blood resin suppressed amplitude of the sodium channel currents in a dose dependent way.
方法：采用全细胞膜片钳记录法，在新鲜分离的大鼠背根神经节细胞上观察血竭对钠通道电流幅值的影响。
Methods The whole cell patch clamp technique was used in isolated ventricular cells of guinea pigs.
方法：用膜片钳实验技术记录胶原酶急性分离的豚鼠心室肌细胞钾通道电流。
The electric physiology feature of L-type calcium channels in the vascular smooth muscle cells was analyzed by patch clamp technique (PCT).
用全细胞模式膜片钳技术记录分析血管平滑肌细胞L型电压依赖性钙通道的特性。
Abstract: the study of lens cells by patch clamp technique began in the early 90 "s."
文摘：应用膜片钳技术对晶体细胞的研究始于90年代初。
MethodsAfter rat hippocampal or cerebral cortical neurons were isolated by pronase e, the IA currents were recorded by whole-cell patch clamp technique.
方法用急性酶解法获得单个大鼠心室肌细胞，标准的全细胞膜片钳技术记录钠通道电流。
Whole cell patch clamp technique was used to record the action potential and ion currents.
以全细胞膜片钳记录单细胞跨膜动作电位和离子电流。
The signal of single ion channel is stochastic ionic currents on the order of 1pA, which can be recorded by means of patch clamp technique.
细胞膜离子单通道信号是皮安级的随机电流，膜片钳技术可以记录这些信号。
Single channel currents were recorded by using the patch clamp technique in inside-out configuration.
急性分离单个的豚鼠气管平滑肌细胞，以膜片钳技术的内面向外式记录单通道电流。
Methods The data was recorded by adopting inside out configuration of patch clamp technique.
方法采用膜片钳技术内面向外式。
Methods The data was recorded by adopting inside out configuration of patch clamp technique.
方法采用膜片钳技术内面向外式。
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