Methods: PCR, gene cloning and successive sub cloning, DNA sequencing, prokaryotic temperature induction, etc. were used.
方法：采用PCR，克隆及连续亚克隆，序列分析，原核温度诱导表达等方法。
Rice Gene Database is one of the key modules of the China National Crop Information Sharing Center, and also one of the sub-systems of Rice Database.
水稻基因数据库系统是在建的国家作物科学数据共享中心的一个重要模块，也是国家水稻数据中心的一个重要子系统。
Objective To sub-clone and express the gene encoding Schistosoma japonicum calcium-binding protein (SJE16) and study its immunological response.
目的亚克隆和表达日本血吸虫钙结合蛋白SjE16的编码基因，初步研究其免疫反应性。
The recombinant plasmid pMM2 contained cholera enterotoxin B sub-unit gene CT-B were expressed in E. coli strain.
本实验室使霍乱肠毒素（CT）B亚单位基因在大肠杆菌中获得表达，并能分泌到胞外。
The recombinant plasmid pMM2 contained cholera enterotoxin B sub-unit gene CT-B were expressed in E. coli strain.
本实验室使霍乱肠毒素（CT）B亚单位基因在大肠杆菌中获得表达，并能分泌到胞外。
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