The allele-specific PCR of the TT1 gene could distinguish purple-leaf mustard from Sichuan Yellow.
通过等位特异PCR可以区分来自四川黄籽与紫叶芥的TT1基因。
Method Several pairs of specific PCR primers were designed according to the conserved region of HDV genome.
方法针对HD V基因保守区域设计多对PCR引物。用芯片点样仪将PCR产物点到玻片上制成基因芯片。
On the basis of cloning and sequencing of OPF02 757 , two PCR primers were designed and a genome specific PCR marker for H.
在对OPF0 2 757进行克隆、测序的基础上,设计一对PCR引物,建立了簇毛麦基因组特异性PCR标记。
United application of STR linkage analysis and multiplex allele specific PCR (MASPCR) in PKU genetic diagnosis was also analysed.
同时分析了STR多态性与多重等位基因特异pcr (MASPCR)在PKU基因诊断中的联合应用。
Aim: To evaluate the clinical value of MSP (methylation specific PCR) in detection of tumor suppressor genes methylation in the colorectal cancer.
目的:评价特异性甲基化pcr (msp)分析法检测抑癌基因高甲基化对结、直肠癌的诊疗价值。
The objective of the present study was to isolate Toxoplasma gondii strains from cats and then to identify these strains by specific PCR assay. The T.
将猫的心、脑、舌用盐酸-胃蛋白酶溶液消化处理后接种小白鼠,经连续传代分离弓形虫虫株,用特异PCR方法对所分离的虫株进行了鉴定。
Species-specific PCR revealed that there were six types of PCR fragments based on their size among the isolates analysed (type 1 to 6) from different continent.
根据种类特异PCR的分析结果,不同大陆的禾谷镰刀菌存在6种类型(类型1-类型6)。
Objective To establish the methylation-specific PCR(MSP) to detect methylation of GTPase regulator associated with the focal adhesion kinase(GRAF) gene promoter.
目的建立甲基化特异性PCR(MSP)技术检测黏着斑激酶相关性鸟苷三磷酸酶调节因子(GRAF)基因启动子甲基化。
Methods a methylation-specific PCR was performed to detect the promoter methylation of RASSF1A gene in 48 tumor tissues, 48 corresponding normal tissues and 48 normal blood plasma.
方法:运用甲基化特异性P CR,检测RASSF1A基因在48例喉癌组织、相对应癌旁组织及48例正常人外周血中的启动子区甲基化情况。
Use of most molecular marker systems depends on the polymerase chain reaction (PCR), which is an important technique for amplifying specific DNA sequences.
大多数分子标记体系的利用都取决于聚合酶链反应(PCR),这是一项扩增特定DNA序列的重要技术。
GlaxoSmithKline and Enigma Diagnostics have an agreement to develop point-of-care PCR tests to identify specific flu strains.
葛兰素史克和英格玛诊断公司联合开发即时PCR试验以鉴别特异流感病毒株。
The technique of PCR could be used to study the infectious and epidemic conditions about MG with its sensitive, quick and specific properties.
P CR技术具有敏感、快速、特异性强特点,可以用来研究MG感染现状,流行情况及致病机制。
The results of genotyping were compared with those obtained with the polymerase chain reaction method using sequence specific primers ( PCR-SSP).
并把分型结果与采用聚合酶链反应-序列特异性引物(PCR-SSP)获得的结果进行比较。
It is revealed in this study that the multiplex PCR, specific, sensitive, rapid, can be applied to routine test of imported fish meal, meat and bone meal.
本研究表明,复合pcr是一种特异、敏感、快速的沙门菌检测方法,可应用于口岸系统鱼粉、肉骨粉的日常检测。
Therefore, the paper suggests PCR amplification DNA is a highly sensitive and specific method for diagnosing tuberculous exudative pleurisy early and rapidly.
提示PCR扩增DNA技术对结核性渗出性胸膜炎是高度敏感和特异的早期、快速诊断方法。
Conclusion: Immuno PCR is a highly specific and sensitive method for the detection of Toxoplasma dissoluble Antigens.
结论:免疫-P CR是一种特异性强,敏感性高的弓形虫可溶性抗原检测方法。
Results: DNA samples from 70 healthy people and 43 patients with psoriasis were detected by PCR with 45 specific primers and two primers as internal quality control.
结果:本研究合成的45条特异性引物和两条内质控引物,检测70例健康人和43例银屑病患者DNA标本。
Objective: to study and establish an allelic specific primer polymerase chain reaction (ASP-PCR) technique system and to apply this technique to study single nucleotide polymorphism (SNP) of genes.
目的:研究建立等位基因特异性引物pcr技术体系,并将其应用于基因单核苷酸多态性研究工作。
Expression of osteoblast specific genes, chondrocyte specific genes, and adipocyte specific genes were confirmed by RT-PCR.
成骨细胞、软骨细胞以及脂肪细胞特异相关或标志基因的表达用RT - P CR检测。
Chapter 2 describes the system part of MPEG-2 standard with focuses on PSI (Program Specific Information) and PCR (Program Clock Reference).
第二章主要讲述了MPEG - 2标准系统层规范,着重介绍节目特殊信息PSI和节目时钟参考pcr。
METHODS:Polymerase chain reaction(PCR) combined with restriction enzyme digest ion were used to detect gene specific junction fragments of the 23 CMT1 patients and 30 normal controls.
方法:应用聚合酶链反应(PCR)-双酶切法,对23例CMT1患者和30例正常人进行基因特异性连接片段的检测。
Method Polymerase chain reaction sequence specific primers (PCR-SSP) method was used to analyze the frequencies of HLA-DQA1 and DQB1 alleles among 189 patients with PV and 273 healthy controls.
方法利用聚合酶链反应-序列特异引物(PCR -ssp)法,对189例银屑病患者和273例健康人的HLA - DQA1和DQB1等位基因进行检测。
Primer is the key of specific reactions of PCR.
引物是PCR特异性反应的关键。
Methods RNA of virus was obtained from samples of cerebrospinal fluid and serum and then gained with specific primer of Enterovirus through RT-PCR so as to detect specific gene fragment.
方法提取患者脑脊液和血清中的病毒rna,用肠道病毒特异性引物进行套式rt - PCR扩增,检测特异性基因片段。
The applications of karyotyping and restriction fragment length polymorphism (RFLP) analyses, specific DNA probes and PCR method to the diagnosis of Candida albicans infections are reviewed.
综述了白色念珠菌感染的分子生物学诊断研究进展,包括核型分析、限制性片段长度多态性分析、特异的DNA探针及PCR等方法。
Objective To screen a species-specific probe of individual bacteria species by PCR fingerprinting, which will facilitate a universal detection system for pathogenic bacteria.
目的利用PCR指纹图技术筛选细菌种特异性探针,探索利用PCR指纹图技术实现病原菌通用检测的可能性。
The experiment results show that the PCR detecting method is specific and sensitive and can be used for detecting and identifying HPS infection.
结果表明,所建立的HPS PCR检测方法特异与敏感性高、适用性强,可应用于HPS感染的诊断和检测。
Methods The distributing frequencies of HLA-DQB1 alleles were detected with polymerase chain reaction-sequence specific primers (PCR-SSP) in 38 GPP patients and 94 healthy subjects from Shandong.
方法运用聚合酶链反应-序列特异性引物(PCR-SSP)法,对38例山东汉族人GPP与94例健康对照进行HLA-DQB1等位基因分型。
Objective: (1) to establish PCR reaction system that USES allele-specific primer PCR technique to detect SNP of KLOTHO gene.
目的:(1)建立使用等位基因特异性引物方法检测KLOTHO基因单核苷酸多态性的PCR反应体系。
Objective: (1) to establish PCR reaction system that USES allele-specific primer PCR technique to detect SNP of KLOTHO gene.
目的:(1)建立使用等位基因特异性引物方法检测KLOTHO基因单核苷酸多态性的PCR反应体系。
应用推荐