T7 and SP6 were taken as sequencing primers for forward and backward sequencing identification automatically.

对其阳性克隆扩大培养，大量提取质粒，以T7，SP6为测序引物进行全自动正、反向测序确认。

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T7 and SP6 were taken as sequencing primers for forward and backward sequencing identification automatically.

对其阳性克隆扩大培养，大量提取质粒，以T7，SP6为测序引物进行全自动正、反向测序确认。

youdao