Genome DNA was extracted from peripheral blood, exon 1 and exon 2 of DAX1 gene were amplified by PCR for sequencing, and mutation screening of SF1 gene (exon 2-7) was conducted.
抽提外周血基因组dna，对DAX1基因2个外显子(外显子1和2)的PCR扩增产物进行测序分析；无突变者行SF 1基因(外显子2 ~ 7)突变筛查。
Genome DNA was extracted from peripheral blood, exon 1 and exon 2 of DAX1 gene were amplified by PCR for sequencing, and mutation screening of SF1 gene (exon 2-7) was conducted.
抽提外周血基因组dna，对DAX1基因2个外显子(外显子1和2)的PCR扩增产物进行测序分析；无突变者行SF 1基因(外显子2 ~ 7)突变筛查。
应用推荐