T7 and SP6 were taken as sequencing primers for forward and backward sequencing identification automatically.

对其阳性克隆扩大培养，大量提取质粒，以T7，SP6为测序引物进行全自动正、反向测序确认。

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The recombinant was sequenced by automatic sequencer with promoters T7 in upstream and T3 in downstream as sequencing primers.

重组体用克隆位点上游和下游的T7和T3启动子序列为测序引物，用自动测序仪测序鉴定克隆的正确性。

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Using normal sequencing primers adjacent to multiple clone sites of plasmid as flanking primers, a novel megaprimer method was performed in one tube with two steps and 34 cycles.

采用大引物方法，利用质粒多克隆位点两侧的普通测序引物作为旁侧引物，在单个PCR管内，经2个步骤共34个循环进行定点突变。

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Using normal sequencing primers adjacent to multiple clone sites of plasmid as flanking primers, a novel megaprimer method was performed in one tube with two steps and 34 cycles.

采用大引物方法，利用质粒多克隆位点两侧的普通测序引物作为旁侧引物，在单个PCR管内，经2个步骤共34个循环进行定点突变。

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