T7 and SP6 were taken as sequencing primers for forward and backward sequencing identification automatically.
对其阳性克隆扩大培养，大量提取质粒，以T7，SP6为测序引物进行全自动正、反向测序确认。
The recombinant was sequenced by automatic sequencer with promoters T7 in upstream and T3 in downstream as sequencing primers.
重组体用克隆位点上游和下游的T7和T3启动子序列为测序引物，用自动测序仪测序鉴定克隆的正确性。
Using normal sequencing primers adjacent to multiple clone sites of plasmid as flanking primers, a novel megaprimer method was performed in one tube with two steps and 34 cycles.
采用大引物方法，利用质粒多克隆位点两侧的普通测序引物作为旁侧引物，在单个PCR管内，经2个步骤共34个循环进行定点突变。
We identified the geminivirus in Malvastrum coromandelianum from the molecular level by designing the primers, PCR, cloning and sequencing.
经设计通用引物、PCR扩增、克隆和测序，首次从分子水平鉴定了杂草赛葵上的双生病毒。
Methods The effects of DNA templates, primers, cycle sequencing reactions as well as purification methods were comparatively analyzed.
方法对测序中模板、引物、测序反应条件及其产物的纯化等因素进行比较研究。
The effects of DNA templates, primers, cycle sequencing reaction conditions, purification methods, operation of instrument were comparatively analyzed.
对测序中的模板、引物、测序反应条件及测序反应纯化方法和仪器操作等进行研究。
MethodsThe corresponding designed primers, RT_PCR, gene cloning, DNA sequencing analysis were used.
方法设计相应的引物，用RT _ PCR，基因克隆，DNA序列分析技术。
Specific primers were designed for MSX1 and PAX9 respectively. Mutation analysis was performed by direct sequencing of all the coding exons and intron-exon boundaries.
分别设计MSX1、PAX9基因特异性引物，聚合酶链反应扩增全部外显子编码区和内含子-外显子剪接序列，产物纯化后直接测序。
Design and search primers for multiple applications including PCR, DNA hybridization and sequencing.
设计和搜索多重引物，包括PCR引物、序列探针和测序引物。
Results:Sequencing failure was caused mainly by DNA template. The ineffective primers could cause sequencing failure.
结果：模板因素是测序成败的主要原因，模板的质量直接影响着测序图谱；
On the basis of cloning and sequencing of OPF02 757 , two PCR primers were designed and a genome specific PCR marker for H.
在对OPF0 2 757进行克隆、测序的基础上，设计一对PCR引物，建立了簇毛麦基因组特异性PCR标记。
On the basis of cloning and sequencing of OPF02 757 , two PCR primers were designed and a genome specific PCR marker for H.
在对OPF0 2 757进行克隆、测序的基础上，设计一对PCR引物，建立了簇毛麦基因组特异性PCR标记。
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