The construction of phage library of scFV antibody.
人源性天然抗体库的构建。
Results The ScFv phage repertoire with capacity of 107 was obtained.
结果获得库容量约为107噬菌体抗体库。
Expression levels of scFv antibodies reached up to 0.5% of total soluble proteins in leaves.
而靶向内质网中表达时，表达量达到总可溶蛋白的0.5%。
Objective: To clone and express humanized mouse ScFv against human cross linked fibrin (HScFv).
目的：克隆和表达人源化小鼠抗人交联纤维蛋白单链抗体。
Multiple alignments with the nearest homologies from the NCBI databases have revealed that the sequences of ScFv obtained are new.
从NCBI的数据库最近的同源性多重路线人士透露，单链抗体的获得的新序列。
To obtain the anti-transferrin receptor scFv and streptavidin(SA) recombinant fusion proteins which were high-efficiently and solubly expressed in E.
拟获得在大肠杆菌中能高效可溶表达的转铁蛋白受体单链抗体与链亲和素（SA）的重组融合蛋白。
Objective to investigate the affects of affinity tag on binding activity of single chain variable fragment (ScFv) to antigen using different immunological assays.
目的探讨在不同的免疫学检测方法中，与单链抗体相连的亲和标记肽对单链抗体与抗原结合活性的影响。
Conclusion Human anti-keratin scFv is obtained from a large phage antibody library, which will benefit to the application of anti-keratin antibody to clinical research.
结论利用噬菌体抗体库技术获得了人源性抗角蛋白单链抗体，为临床应用研究提供了具有更广阔应用前景的人源小分子抗体。
AIM: To obtain in situ pairing of the variable region genes of the heavy and light chains of vitiligo patients' lymphocytes, then construct and screen a human ScFv phage antibody library.
目的克隆白癜风患者淋巴细胞中原始配对的自身抗体基因，构建噬菌体抗体库并筛选针对黑素细胞膜抗原的特异性抗体。
AIM: To obtain in situ pairing of the variable region genes of the heavy and light chains of vitiligo patients' lymphocytes, then construct and screen a human ScFv phage antibody library.
目的克隆白癜风患者淋巴细胞中原始配对的自身抗体基因，构建噬菌体抗体库并筛选针对黑素细胞膜抗原的特异性抗体。
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