Because now there's minus t divided by RT squared.
现在又负T除以rt平方。
PCR and RT PCR showed that CD gene was transferred into the t lymphocyte and expressed successfully.
经pcr和RT PCR方法检测证明CD基因已成功地导入T淋巴细胞中并有效表达。
To study dysfunction of activated T cell in peripheral blood and make clear its role on pathogenesis in patients with recurrent tonsillitis(RT).
目的探讨反复扁桃腺炎（RT）患儿T细胞活化功能障碍情况，为分析RT的病因和发病机制提供理论依据。
Methods RT PCR products were amplified from S85 46 virus infected cells, cloned into T vector, sequenced and analyzed using DNASTAR software.
方法将特异性引物从S8546病毒感染细胞PCR扩增的产物克隆于T载体，正确的克隆纯化后测序，应用DNASTAR软件比较分析。
Further the earth pressure distribution with RB and rt modes was obtained using the same software, and the results were compared with t mode.
进一步研究了RB、RT模式挡土墙土压力的分布情况，并与T模式情况进行对比。
Methods The L and M segment cDNA of Hantavirus Q32 strain was amplified by RT-PCR. The purified PCR products were sequenced directly or cloned into pGEM-T Vector and then sequenced.
方法设计特异的PCR引物，用RT-PCR技术分段扩增Q32株全长L、M片段，PCR产物纯化后直接测序，或用T-A克隆方法进行PCR产物克隆，然后进行遗传进化分析。
Methods The L and M segment cDNA of Hantavirus Q32 strain was amplified by RT-PCR. The purified PCR products were sequenced directly or cloned into pGEM-T Vector and then sequenced.
方法设计特异的PCR引物，用RT-PCR技术分段扩增Q32株全长L、M片段，PCR产物纯化后直接测序，或用T-A克隆方法进行PCR产物克隆，然后进行遗传进化分析。
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