The expression of E7 gene was examined by RT PCR and the indirect immunofluorescence.
E7基因表达的检测采用RT PCR和间接免疫荧光实验。
After sequence analysis, RT PCR method was used to analyze the expression pattern in human fetal tissues.
序列分析后用RT PCR方法研究胎儿组织中的表达情况。
Methods HGV RNA in plasma of 84 IVDUs was detected by reverse transcription polymerase chain reaction (RT PCR).
方法采用逆转录聚合酶链反应(RT - PCR)检测84例静脉毒瘾者血浆标本。
ConclusionDue to specificity on enterovirus, the RT PCR method has some value in the diagnosis of enterovirus infections.
结论该RT -PCR方法具有肠道病毒特异性，在肠道病毒感染的诊断中有一定应用价值。
Methods RT PCR products were amplified from S85 46 virus infected cells, cloned into T vector, sequenced and analyzed using DNASTAR software.
方法将特异性引物从S8546病毒感染细胞PCR扩增的产物克隆于T载体，正确的克隆纯化后测序，应用DNASTAR软件比较分析。
The result showed that PRRSV can be quickly diagnosed by RT PCR, which laid a basis for quick diagnosis of PRRS and further research of the PRRSV isolates of our country.
结果说明应用所设计的引物进行RT PCR快速检测PRRS是可行的，为我国快速特异诊断PRRS和PRRSV强毒株的深入研究奠定了基础。
Objective To increase the sensitivity of quantitative detection of gene transcripts in peripheral blood using real time RT PCR, two specimen processing methods were compared.
目的选择合理的标本处理方法，提高外周血基因转录本检测的灵敏度。
Quantitive RT PCR was used to observe the effect of repeated acute hypoxic exposure on the expression of glucose transporter protein 1 and 3 genes and tolerance in the mice brain.
采用定量反向转录-聚合酶链式方法，观察急性重复性低氧对小鼠脑内葡萄糖转运蛋白1，3基因表达的影响。
One step nested reverse transcriptase-polymerase chain reaction (RT-PCR) was used.
使用一步法巢式逆转录聚合酶链扩增法(RT - PCR)。
Objective to evaluate the clinicopathologic significance of the detection of peritoneal micrometastases in gastric cancer by reverse transcriptase-polymerase chain reaction (RT-PCR).
目的评估用逆转录聚合酶链式反应(RT PCR)方法检测胃癌腹腔微转移的临床病理意义。
MethodsReverse Transcription-polymerase chain reaction(RT-PCR) was used.
方法采用逆转录-聚合酶链反应（RT-PCR）方法。
Methods 40 patients with lung cancer were studied to detect TTF-1 gene by using reverse transcription-polymerase chain reaction assay (RT-PCR).
方法采用逆转录—聚合酶链反应(RT -PCR)方法检测40例患者的肺癌组织ttf - 1基因的表达。
Methods: the blood samples were collected from HFRS patients between 2000 ~ 2001, and the m segment genome of hantavirus was amplified by RT-PCR.
方法：采集2000 ~ 2001年肾综合征出血热(HFRS)患者的血液标本，通过逆转录聚合酶链反应(RT - PCR)扩增汉坦病毒M片断基因。
Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to measure the mRNA levels of active efflux gene CDR1 and CDR2.
用逆转录-聚合酶链反应(RT - PCR)方法检测主动外排泵基因CDR1和CDR2的表达水平。
The gene expression of ET-1, ETAR, ETBR and ECE was evaluated by semi-quantitative reverse transcription polymerase chain response (RT-PCR).
采用半定量逆转录多聚酶链反应(RT - PCR)检测局部内皮素系统ET - 1、ETAR、ETBR及ECE的基因表达。
Semiquantitative analysis of IL-6 in the hippocampus was done at different time and dose level after whole-brain irradiation with reverse-transcription polymerase chain reaction (RT-PCR).
应用逆转录聚合酶链反应(RT - PCR)半定量分析大鼠脑放射性损伤后海马区在不同时间、不同剂量水平IL - 6基因转录的动态表达。
Methods: The expression of VEGF gene in 48 samples of human gastric carcinomas and 30 samples of corresponding normal tissue specimens was detected by RT-PCR.
方法：采用逆转录-聚合酶链反应(RT - PCR)检测48例胃癌组织及30例正常组织中vegf基因的表达。
Methods RT-PCR was applied to study expression of MRP gene in tumor tissues from 83 cases of esophageal and cardiac carcinoma, and 45 lymph nodes from 28 patients.
方法应用逆转录酶-多聚酶链反应(RT - PCR)方法，检测了83例食管癌和贲门癌组织及28例患者45枚淋巴结中MRP基因的表达，并与相应癌旁组织进行对照分析。
METHODS: We used RT-PCR, flow cytometry and immunofluorescence cell staining techniques to observe the expression of PARs on A549 cells.
方法：采用逆转录聚合酶链反应(RT - PCR)、流式细胞仪和免疫荧光细胞染色分析技术分析肺上皮细胞系a549细胞PAR s的表达情况。
Relationship between RT-PCR and rabbit-cross test for detection of classical swine fever virus (CSFV) was studied in this experiment.
对猪瘟RT PCR诊断方法与兔体交叉试验诊断方法的相关性进行了研究。
Quantitative RT-PCR results indicated representation of OsBTB gene expression was corresponding with the selected resistant and pathogenesis-related genes.
定量PCR结果表明该基因的表达模式与所选取的抗性及抗性相关基因的表达模式一致。
Myrosinase activity was detected in all the tested organs except for pulp, which is consistent with the result of RT-PCR.
酶活性测定表明，除了果肉外的其它器官中都有芥子酶活性，这与RT-PCR的结果是一致的。
The level of promiscuous gene expression was detected by RT-PCR.
利用RT - PCR方法检测小鼠胸腺异位基因表达水平。
Methods RNA from human peripheral blood mononuclear cells were extracted and target genes were amplified by RT-PCR.
方法提取人外周血单个核细胞总RNA，反转录聚合酶链反应（RT-PCR）扩增目的基因；
The intracellular pattern recognition receptor NOD1 is detected in human lung epithelial cells by RT-PCR.
并用RT-PCR的方法检测肺上皮细胞胞内模式识别受体NOD1的表达。
The results of RT-PCR analysis for 7 differently expressed genes were coincident with those of microarray assay.
PCR技术对其中7个基因表达差异的验证结果与基因芯片结果一致。
Analysis of RT-PCR and sequencing indicated that the ncRNA gene had two different transcripts through alternatively splicing pattern.
PCR和测序结果分析，发现这个非编码RNA基因通过选择性剪接产生两种不同的转录产物。
Analysis of RT-PCR and sequencing indicated that the ncRNA gene had two different transcripts through alternatively splicing pattern.
PCR和测序结果分析，发现这个非编码RNA基因通过选择性剪接产生两种不同的转录产物。
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