The expression of E7 gene was examined by RT PCR and the indirect immunofluorescence.
E7基因表达的检测采用RT PCR和间接免疫荧光实验。
Methods Expression of ABC family genes was examined by RT PCR, and cell growth by MTT test.
方法应用rtP CR检测abc家族基因的转录表达，MTT法检测耐药性改变。
Methods HGV RNA in plasma of 84 IVDUs was detected by reverse transcription polymerase chain reaction (RT PCR).
方法采用逆转录聚合酶链反应(RT - PCR)检测84例静脉毒瘾者血浆标本。
Methods: The expression of B7 in 22 cases of renal cell carcinoma was studied by RT PCR and immunochemical methods.
方法：本文应用rt ？PCR和免疫组织化学技术检测了22例肾细胞癌组织B7的表达。
ConclusionDue to specificity on enterovirus, the RT PCR method has some value in the diagnosis of enterovirus infections.
结论该RT -PCR方法具有肠道病毒特异性，在肠道病毒感染的诊断中有一定应用价值。
Viral detection by reverse transcription polymerase chain reaction (RT-PCR) assay, and.
采用逆转录聚合酶链式反应(RT - PCR)检测病毒。
RT–PCR products from clinical samples may also be used for genotyping of the virus, allowing comparisons with virus samples from various geographical sources.
临床样品RT-PCP制品也可用于病毒基因分型，与采自不同区域的病毒取样做出比较。
Methods Using reverse transcription-polymerase chain reaction (RT-PCR), the PIM3 mRNA expression in normal murine ocular tissues was defected.
方法应用逆转录-聚合酶链反应及免疫组化检测PIM3在小鼠眼部组织中的表达。
Quantitative RT-PCR results indicated representation of OsBTB gene expression was corresponding with the selected resistant and pathogenesis-related genes.
定量PCR结果表明该基因的表达模式与所选取的抗性及抗性相关基因的表达模式一致。
The result indicated that fluorogenetic quantitation RT-PCR may be used as a good reference in detection of H5N1 TIV.
这说明荧光定量RT-PCR方法可以对临床上H5N1虎源流感病毒检测提供参考。
Methods MDR1 gene expression in case of 30 leukemia and 8 healthy persons' peripheral blood have been detested by fluorescence-quantitative reverse transcription-polymerase chain reaction (RT-PCR).
方法应用荧光定量逆转录-多聚酶链反应（RT -PCR）检测了30例急性白血病患者和8例正常人外周血MDR1基因的表达。
Objective to evaluate the clinicopathologic significance of the detection of peritoneal micrometastases in gastric cancer by reverse transcriptase-polymerase chain reaction (RT-PCR).
目的评估用逆转录聚合酶链式反应(RT PCR)方法检测胃癌腹腔微转移的临床病理意义。
One step nested reverse transcriptase-polymerase chain reaction (RT-PCR) was used.
使用一步法巢式逆转录聚合酶链扩增法(RT - PCR)。
Methods 40 patients with lung cancer were studied to detect TTF-1 gene by using reverse transcription-polymerase chain reaction assay (RT-PCR).
方法采用逆转录—聚合酶链反应(RT -PCR)方法检测40例患者的肺癌组织ttf - 1基因的表达。
Methods: the blood samples were collected from HFRS patients between 2000 ~ 2001, and the m segment genome of hantavirus was amplified by RT-PCR.
方法：采集2000 ~ 2001年肾综合征出血热(HFRS)患者的血液标本，通过逆转录聚合酶链反应(RT - PCR)扩增汉坦病毒M片断基因。
These RT-PCR products were sequence verified.
这些RT - PCR扩增产物的序列验证。
MethodsReverse Transcription-polymerase chain reaction(RT-PCR) was used.
方法采用逆转录-聚合酶链反应（RT-PCR）方法。
Myrosinase activity was detected in all the tested organs except for pulp, which is consistent with the result of RT-PCR.
酶活性测定表明，除了果肉外的其它器官中都有芥子酶活性，这与RT-PCR的结果是一致的。
Methods RNA from human peripheral blood mononuclear cells were extracted and target genes were amplified by RT-PCR.
方法提取人外周血单个核细胞总RNA，反转录聚合酶链反应（RT-PCR）扩增目的基因；
The intracellular pattern recognition receptor NOD1 is detected in human lung epithelial cells by RT-PCR.
并用RT-PCR的方法检测肺上皮细胞胞内模式识别受体NOD1的表达。
Results: the linear amplification range of PCR and the best cycle number and template of RT-PCR were obtained.
结果：获取了PCR线性扩增范围，确定了RT - P CR的最佳循环次数和模板量。
The results of RT-PCR analysis for 7 differently expressed genes were coincident with those of microarray assay.
PCR技术对其中7个基因表达差异的验证结果与基因芯片结果一致。
Mutation analysis was detected by direct sequencing RT-PCR products.
直接测序法检测RT PCR产物的基因变异；
Analysis of RT-PCR and sequencing indicated that the ncRNA gene had two different transcripts through alternatively splicing pattern.
PCR和测序结果分析，发现这个非编码RNA基因通过选择性剪接产生两种不同的转录产物。
Analysis of RT-PCR and sequencing indicated that the ncRNA gene had two different transcripts through alternatively splicing pattern.
PCR和测序结果分析，发现这个非编码RNA基因通过选择性剪接产生两种不同的转录产物。
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