Because different tissues of same plant species differ in component composition, RNA extraction methods have different nodi.
同种植物不同器官的组织由于组成分的差异，提取RNA的方法也存在不同的难点。
The strategic and tactical advantages, principle, screening methods, achievements of RNA as a small molecule drug target were reviewed.
对RNA药靶的特点、研究策略和针对RNA药靶的小分子药物筛选方法，进行了综述。
Conclusion: the results suggest that it had significant difference between four RNA extraction methods, so we should choose the appropriate one for detection of flu viruses in clinical specimens.
对临床标本的检测，也获得相似结果。结论：各试剂之间提取核酸的效率存在差异，应该选择性价比较好的方法进行临床标本的实验室RNA提取。
Two total RNA extraction methods were developed firstly.
建立了两种木本植物总rna的提取方法。
In this article the principle and methods in constructing RNA replicon-based vectors of Flavivirus virus their applications were reviewed.
本文综述黄病毒属病毒复制子的构建原理、方法及应用。
The methods for RNA secondary structure prediction can be divided into two categories which are the methods based on thermodynamics and phylogenetics.
RNA二级结构预测方法可以分为基于热力学的预测方法和基于系统发生学的预测方法两大类。
There are many methods for extracting RNA from brewing yeast. The salt and the alkali are often used.
从啤酒酵母中提取核酸的方法有很多，常用的是盐法和碱法。
Objective: To investigate the methods of the collection of samples of fish tissue and blood and the extraction of RNA and compare with the other methods.
前言：目的：探讨从鱼组织、血液样本的采集及提取rna的方法和其他方法的比较。
Furthermore, the present invention provides methods for suppressing cancer, which comprise the step of administering a minus-strand RNA virus to cancer tissues.
另外，本发明还提供了包含将单链负链rna病毒向癌组织进行给药的步骤的癌症抑制方法。
Furthermore, the present invention provides methods for producing the anticancer agents, which comprise the step of mixing a minus-strand RNA virus with pharmaceutically acceptable carriers.
另外，本发明还提供了包含将单链负链rna病毒和药学上可容许的承运体混合的步骤的抗癌剂的制备方法。
Objective To establish a super-sensitive diagnosis method for influenza a (H1N1) by enrichment of virus RNA using several settling agents and nucleic acid purification methods.
目的利用几种助沉剂和核酸纯化方法富集病毒rna，建立甲型H1N 1流感超敏感诊断方法。
The quality and quantity of extracted RNA were compared among these methods.
并将提取效果最好的方法应用于临床标本。
The method could produce cotton total RNA with higher purity and integrality in a shorter time from different tissues of cotton than other conventional methods.
该方法与异硫氰酸胍法或冷酚法等相比具有更简便、得到的棉花组织总rna完整性好和纯度高等优点。
RNA interference methods and gene inducible expression techniques to use on study of gene regulations in cells are transferred to making transgenic animals, and the ideal research results are gained.
用于细胞基因调控研究的RNA干涉技术和基因诱导表达方法，现在移植到了转基因动物中，并获得了理想结果。
Three methods were compared in the extraction of total RNA from Birch leaves. CTAB methods were the ideal for the trees?
比较了三种树木RNA的提取方法，确定改良的CTAB法为白桦组织RNA的最优的提取方案。
Methods HGV RNA in plasma of 84 IVDUs was detected by reverse transcription polymerase chain reaction (RT PCR).
方法采用逆转录聚合酶链反应(RT - PCR)检测84例静脉毒瘾者血浆标本。
Methods RNA from human peripheral blood mononuclear cells were extracted and target genes were amplified by RT-PCR.
方法提取人外周血单个核细胞总RNA，反转录聚合酶链反应（RT-PCR）扩增目的基因；
Methods Dual-electrode voltage clamp technique was used to record membrane currents from follicular oocytes, and defolliculated oocytes that had been injected with alien RNA and incubated.
方法应用双电极电压钳技术分别在带滤泡膜及去滤泡膜后注入RNA并经孵育的卵母细胞记录膜电流。
Methods Non radioactive RNA in situ hybridization.
方法非放射性RNA原位杂交。
With tea plant embryos as the research object, we compares and analyses several methods of total RNA extraction.
茶树种胚中富含多糖和多酚类化合物，这增加了总RNA的提取难度。
METHODS Total RNA was extracted from human embryonic lung fibroblast cells (WI 38) and the whole length gene of human OPG was obtained by RT PCR.
方法从培养的人胚肺成纤维细胞(WI- 38)中提取总rna，经rt -PCR获得人破骨细胞抑制因子基因。
Methods RNA of virus was obtained from samples of cerebrospinal fluid and serum and then gained with specific primer of Enterovirus through RT-PCR so as to detect specific gene fragment.
方法提取患者脑脊液和血清中的病毒rna，用肠道病毒特异性引物进行套式rt - PCR扩增，检测特异性基因片段。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR product was cloned into t vector, sequenced and analyzed using DNASTAR software.
方法从Z 10株病毒感染的细胞提取总rna，将逆转录pcr扩增的产物纯化后克隆于t载体并进行序列测定，应用dnastar软件分析比较。
Methods HCV RNA positive samples were analyzed by both gene chip and line probe assay in a comparative analysis.
方法对HCVRNA阳性血清用基因芯片法和线性探针法进行基因分型。
Methods Histochemistry, in situ hybridization and intact cell RNA dot blot techniques were used.
方法组织化学、原位杂交和完整细胞RNA斑点印迹。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR products was cloned into t vector, sequenced and analyzed by using DNASTAR software.
方法从Z 10病毒感染的细胞提取总rna，将逆转录聚合酶链反应(RT PCR)扩增的产物纯化后克隆于t载体并进行序列测定，应用dnas TAR软件分析比较。
Objective To study biochemical function and therapy of the NP (nucleocapsid protein) gene of NDV (new castle disease virus). Methods the purified genome RNA of NDV were used for template.
目的研究新城疫病毒(NDV)核衣壳蛋白(NP)基因的生物学作用及其抗人喉鳞癌的作用。
Methods the total RNA was extracted from Z37 virus infected cells and the RT-PCR products were cloned into t vector, sequenced and analyzed by DNASTAR software.
方法设计出针对各片段的特异性引物，用P CR方法从Z 37病毒感染的细胞提取细胞总rna，逆转录扩增、产物克隆t载体，纯化后测序，测定的序列应用DNASTAR软件比较分析。
Methods Thirty cases of hepatic cirrhosis and ascites were treated with injection of RNA for 2 months. Meanwhile, the results were compared with those in other comprehensively treated 30 cases.
方法使用注射用核糖核酸治疗肝硬变重度腹水30例，疗程2月，并与综合治疗组对比分析。
Methods the 64 serum samples were collected from patients of hemorrhagic fever with renal syndrome (HFRS), and the virus RNA was extracted as pattern.
方法采集64例肾综合征出血热(HFRS)急性期患者血清标本，提取血清中病毒RNA作为模板。
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