The underscores were the sites for restriction digest.
下划线是限制性酶切位点。
The partial digest (PDP) and the simple partial digest (SPDP) methods are the popular methods of DNA restriction mapping reform.
DNA限制性图谱绘制常用方法有部分消化法（PDP）和简化的部分消化方法（SPDP）。
METHODS:Polymerase chain reaction(PCR) combined with restriction enzyme digest ion were used to detect gene specific junction fragments of the 23 CMT1 patients and 30 normal controls.
方法：应用聚合酶链反应（PCR）-双酶切法，对23例CMT1患者和30例正常人进行基因特异性连接片段的检测。
METHODS:Polymerase chain reaction(PCR) combined with restriction enzyme digest ion were used to detect gene specific junction fragments of the 23 CMT1 patients and 30 normal controls.
方法：应用聚合酶链反应（PCR）-双酶切法，对23例CMT1患者和30例正常人进行基因特异性连接片段的检测。
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