Specific primers were designed for MSX1 and PAX9 respectively. Mutation analysis was performed by direct sequencing of all the coding exons and intron-exon boundaries.
分别设计MSX1、PAX9基因特异性引物，聚合酶链反应扩增全部外显子编码区和内含子-外显子剪接序列，产物纯化后直接测序。
The titer of the antiserum was respectively 1:256 by agarose double-diffusion analysis and it was 1:12800 by indirect ELISA, specific antisera against the fusion protein were prepared.
琼脂糖双向扩散检测显示抗血清的效价1：25 6，间接ELISA测得的效价1：12 800，结果显示获得了融合蛋白的特异性抗血清。
The titer of the antiserum was respectively 1:256 by agarose double-diffusion analysis and it was 1:12800 by indirect ELISA, specific antisera against the fusion protein were prepared.
琼脂糖双向扩散检测显示抗血清的效价1：25 6，间接ELISA测得的效价1：12 800，结果显示获得了融合蛋白的特异性抗血清。
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