Optimization of the renaturation conditions for recombinant human proinsulin inclusion bodies expressed in E.
对大肠杆菌表达的重组人胰岛素原包涵体蛋白的变性复性条件进行了优化。
The process of renaturation is established in the understanding to the mechanism of protein folding in vitro.
变复性实验是建立在对蛋白质体外折叠机制的了解的基础上。
After purification, the target protein further renaturation 2 - BMP via degeneration dissolving, load dialysis bag.
纯化后的的目的蛋白进一步复性，BMP-2经变性液溶解，装入透析袋内。
The renaturation of gene recombinant protein is the most important technique in the field of biotechnology which's many methods have been established.
基因重组蛋白质的复性是当前生物工程领域的重要研究课题。
Fusion protein expression was induced by IPTG. After renaturation, the protein was purified by affinity chromatography and the bioactivity was examined by ELISA.
IPTG诱导表达融合蛋白，包涵体蛋白经复性后亲和层析法纯化，ELISA方法测定蛋白活性。
The recombination fusion protein was purified by lysis, washing, denaturation and renaturation. The specific band of expression was excised from the gel and used to immunize mice .
通过裂解、洗涤、变性、复性等方法对包涵体蛋白进行处理，以电泳切胶回收所获得的纯化产物作为抗原，免疫小鼠制备抗重组蛋白血清。
After the peptide chain has been unfolded by heating, by exposure to low pH, or by treatment with urea, it will often spontaneously refold to the native form, this sequence is called renaturation.
肽链加热打开之后，将其置于低pH值环绕或用尿素处理，再折叠到原来的形状，这个过程叫做复体。
The kinetics of the renaturation and reactivation of glucoamylase denatured by guanidine HCl was determined by means of the UV differential absorbance measurement and fluorescence emission spectra.
本文利用荧光、紫外差光谱研究了根霉葡萄糖淀粉酶在盐酸胍变性后的复性、复活动力学。
Objective To examine the changes of conformation and activity of human placental alkaline phosphatase(HPAP) in denaturation and renaturation of guanidine chloride, and to study its folding mechanism.
目的观测人胎盘碱性磷酸酶（HPAP）在盐酸胍变性和复性过程中构象与活力的变化，探讨其折叠机制。
Objective To examine the changes of conformation and activity of human placental alkaline phosphatase(HPAP) in denaturation and renaturation of guanidine chloride, and to study its folding mechanism.
目的观测人胎盘碱性磷酸酶（HPAP）在盐酸胍变性和复性过程中构象与活力的变化，探讨其折叠机制。
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