Objective to evaluate the clinical application of fluorescent quantification polymerase chain reaction (FQ PCR) to detect cytokeratin 19 (CK19) in the diagnosis of breast cancer.
目的探讨荧光定量pcr (FQPCR)检测细胞角蛋白19 (ck19)在乳腺癌诊断中的应用。
The result indicates that the CRT-PCR is a convenient device to enhance the sensitivity of RNA detection and can be applied in the quantification of RNA.
结果提示，CRT -PCR是一种简便有效的提高RNA检测灵敏度的方法，并适合应用于定量分析。
Methods These three genes were quantified by real-time quantification polymerase chain reaction (PCR) in er positive and er negative breast cancer cell lines.
方法应用实时监测聚合酶链反应检测这三种受体基因在雌激素受体阳性和雌激素受体阴性乳腺癌细胞系中的表达。
Results showed that 1. fluorescent quantitative RT-PCR was an efficient quantification method to detect the gene expression level and has a prospecting application in clinic;
结果显示，1．荧光定量RT干CR方法是一种定量检测基因表达丰度的有效方法，具有很好的临床应用前景；
Results showed that 1. fluorescent quantitative RT-PCR was an efficient quantification method to detect the gene expression level and has a prospecting application in clinic;
结果显示，1．荧光定量RT干CR方法是一种定量检测基因表达丰度的有效方法，具有很好的临床应用前景；
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