Its RNA polymerase activity was dependent upon the primer in vitro with limited reverse transcription activity.
其RNA依赖的RNA聚合酶活性有一定的引物依赖性。
This review summarized the recently research process of Sq RT-PCR. It including extraction of total RNA, primer design, definition of cycle count, selection of inner reference and so on.
该文就半定量RT-PCR法的检测步骤及技术的关键因素（总RNA提取、引物设计、循环数的确定和内参的选择等）进行综述。
Methods RNA of virus was obtained from samples of cerebrospinal fluid and serum and then gained with specific primer of Enterovirus through RT-PCR so as to detect specific gene fragment.
方法提取患者脑脊液和血清中的病毒rna，用肠道病毒特异性引物进行套式rt - PCR扩增，检测特异性基因片段。
One version of the dideoxy sequencing method can be used to sequence the 5'ends of RNA molecules by 'primer extension'.
一种形式的双脱氧序列测定法是通过引子的延长来测定RNA分子5’末端的序列。
The primer was designed based on consensus sequence from RRM RNA binding protein gene family. The results showed that there were stable expressed fragments among the different mRNA samples.
不同材料间具有稳定表达的组成型RRMRNA结合蛋白基因，同时具有丰富的差异表达的具调控功能的RRM RNA结合蛋白基因。
The primer was designed based on consensus sequence from RRM RNA binding protein gene family. The results showed that there were stable expressed fragments among the different mRNA samples.
不同材料间具有稳定表达的组成型RRMRNA结合蛋白基因，同时具有丰富的差异表达的具调控功能的RRM RNA结合蛋白基因。
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