Objective:To explore a simple and reliable technique to keep positive DNA controls.
目的：探索能获得阳性DNA对照的简便可靠的方法。
We applied the sister chromatid exchange (SCE) technique to pregnant women with antibodies against cytomegalovirus (CMV) positive, to detect the lesion and repair of DNA in the lymphocytes.
采用孕妇外周血淋巴细胞姐妹染色单体交换（SCE）技术，检测巨细胞病毒（CMV）抗体阳性孕妇淋巴细胞DNA损伤和修复。
Meanwhile, biochemical reaction, coagglutination test, metabolism-inhibition test, polymerase chain reaction (PCR), and DNA sequence assay were employed to identify those positive cultures.
阳性培养物用生化反应、协同凝集试验、代谢抑制试验、聚合酶链反应和DNA测序来加以鉴定。
Methods HBV genotypes were detected by polymerase chain reaction (PCR) and restriction fragment length polymorphism analysis (RFLP) in 136 HBV DNA positive patients who were born in Shanghai.
方法采用聚合酶链反应-限制性片段长度多态性分析(PCR - RFLP)检测136例HBVDNA阳性的上海籍HBV感染者的基因型。
Plasmid DNA and chromosomal DNA of the strain CEOI all obtained positive dot-blot to plasmid probe and chromosomal probe correspondingly.
质粒dna和染色体dna分别与质粒探针和染色体探针杂交出现典型的阳性杂交斑。
Objective to observe the therapeutic effect of autologous cytokine-induced killer cells (CIK) on HBV DNA positive patients with liver cirrhosis.
目的观察自体细胞因子诱导杀伤细胞(CIK细胞)治疗HBVDNA阳性肝硬化患者的近期疗效。
All the 6 genotypes (9 strains) of measles virus showed DNA positive strand, it means this RT-PCR method is sensitive for measles viruses.
结果9株6种基因型麻疹病毒RT PCR均呈现阳性条带，说明本研究采用的RT PCR方法敏感。
The positive control cell line DNA was mixed in different proportions with the DNA extracted from reactive lymphoid tissue to test the sensitivity of the touch-down PCR.
阳性细胞系DNA模板与反应增生性淋巴组织DNA按不同比例混合后扩增，检测降落式PCR的灵敏度。
Objective To study the effect of HBV-DNA positive serum on phenotypic transformation of renal tubular epithelial cells.
目的研究HBV - DNA阳性血清对肾小管上皮细胞表型转化的作用。
Objective To acknowledge the existence and replication of TTV DNA in peripheral blood mononuclear cells(PBMC) of patients with serum TTV DNA positive.
目的了解TTV阳性肝炎患者外周血单个核细胞（PBMC）TTVDNA存在及复制情况。
Sandwich ELISA, dot blotting and DNA sequencing methods were used for the identification of positive clones.
通过硝酸纤维膜斑点印迹法进行阳性克隆鉴定。
Results: 39 out of 41 HCMV-DNA positive samples were identified positive and all of the 232 HCMV-DNA negative samples were negative by this method.
结果：41例证实为HCMV - DNA阳性标本中该产前诊断方法检测出39例，该方法对232例HCMV - DNA阴性标本诊断均为阴性。
Methods To introduce DNA encoding the wild- type and mutant channel into immortalized tyrosine hydroxylase- positive CNS- derived neurons, CAD cells.
方法给一类酪氨酸脱氢酶阳性的神经细胞（一种中枢神经源细胞），又称CAD细胞，引入由DNA编码的野生型和突变型离子通道。
Specific antibody was screened by 3 rounds of panning of phage antibody library with the fusion protein. The antigen binding activity and DNA sequences of positive clones were determined and analyzed.
以该融合蛋白为固相抗原，对噬菌体抗体库进行3轮淘筛，并对所获阳性克隆进行抗原结合活性测定和DNA序列测定。
Provides complete reagents including positive and negative control slides for convenient detection of DNA fragmentation in tissue sections and fixed cell preps. Results can be analyzed by microscopy.
提供完整的组份，包括阳性，阴性对照组织切片，便于检测组织切片和固定细胞中的片段化dna，结果可通过显微镜分析。
Also provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells by flow cytometry.
该试剂盒也提供完整的组份，包括阳性，阴性对照细胞，便于采用流式细胞术检测培养细胞中的片段化dna。
Result in the PCR amplification sensitivity testing revealed that 0.021 egg DNA template would be enough to amplify this specific positive band.
其PCR扩增敏感性检测结果表明，0.021个虫卵dna模板即可扩增出该特异性阳性条带。
TB-DNA was detected in 8 of 41 malignant tumor tissues (19 5%), moreover, the incidence locations of TB-DNA positive malignant tumors were mostly similar to that of tuberculosis.
结果41例恶性肿瘤组织中检出TB - dna阳性患者8例，占19.5 %，且TB - dna阳性患者肿瘤发病部位基本与结核病的好发部位一致。
The appearance of double bands in several inbred lines by some primers was probably caused by electrophic difference between positive and negative DNA chains.
在试验中某些引物在亲本自交系中出现双带，分析认为可能是由于正负链电泳行为差异造成的。
Objective To study the clinical application of DNA chip for common Gram-positive bacteria identification and drug resistance detection.
目的利用基因芯片技术进行常见革兰阳性细菌的细菌鉴定和耐药性检测。
HPV DNA testing already has a poor positive predictive value, especially in younger girls, they point out, and the absolute number of true positive tests would decrease substantially in this scenario.
他们指出，HPVDNA检验的阳性预测值已经不高，特别是对年轻的女性，在这种情况下，真阳性检验的绝对数目将会显著降低。
DNA sequence assays showed that 5 of the 6 positive clones displayed the same peptide sequences.
DNA测序结果显示，所得到的6个噬菌体阳性克隆中，有5个阳性克隆递呈相同的氨基酸序列。
Aim To explore the effect lamivudine (LMV) on the liver function of patients with HBV DNA positive pulmonary tuberculosis.
目的探讨拉米呋啶(LMV)对HBVDNA阳性肺结核患者肝功能干预作用。
That implies that these regions are so important for the organism that their DNA sequences are maintained by positive selection.
这意味着这些区域对于机体如此重要以至于它们的DNA序列通过主动选择来保持。
Results For 18 nasopharyngeal carcinoma patients, positive rates of EB virus DNA in plasma and leucocytes were 61%(11/18);
结果18例鼻咽癌患者外周血血浆和白细胞检测EB病毒DNA的阳性率相同，均为61%（11/18）；
The positive cells were selected by G418, genome DNA of the positive cells was extracted and sequenced.
经g 418筛选阳性细胞，提取阳性细胞的基因组d NA并测序。
Results: Reliable detection of DNA methylation of TLX3 and FOXE3 segregated ALL from those clear of disease with minimal false-negative and false-positive results.
结果：在最小限度的假阴性及假阳性结果中，TLX3和FOXE3DNA甲基化检测可有效鉴别ALL。
Methods:PCR was used to detect HPV16 DNA in 67 samples of cervical carcinoma, the protein expression of HPV16 E7 were examined in HPV16 DNA positive samples through immunohistochemical ABC method.
方法：运用PCR技术探查67例宫颈癌组织中HPV16感染率，并运用免疫组化ABC法检测HPV16感染对宫颈癌中E7蛋白表达情况。
In a statement the council said: "Lancashire County council has withdrawn a beef product from 47 school kitchens after it provisionally tested positive for traces of horse DNA."
地方议会在声明中说：“在初步检测含有马肉后，兰开夏郡地方议会已回收了47所学校食堂中所谓的牛肉食品。”
Methods Genomic DNA was extracted from the whole blood samples collected from HIV-1 positive patient. Nested PCR was carried out and PCR products were purified and used directly for sequencing.
方法从确诊的HIV - 1感染者全血样本中提取基因组dna，经套式聚合酶链反应(PCR)扩增和测序。
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