The construction of phage library of scFV antibody.
人源性天然抗体库的构建。
Phage-displayed peptide library technology is based on expressing recombinant random peptides on the surface of filamentous phage and selecting function peptides.
噬菌体展示技术是将随机多肽文库表达于丝状噬菌体的表面，筛选功能性多肽的一种生物技术。
Objective:To obtain the mimotope of avian infectious bronchitis virus by phage display peptide library.
目的：利用噬菌体展示肽库技术筛选鸡传染性支气管炎病毒的模拟抗原表位。
Phage displayed technology has become a powerful tool in preparing high affinity antibodies, and antibody library technology paves a way for obtaining high affinity antibodies.
噬菌体展示技术是一种制备高亲和力抗体的有力工具，抗体库技术则为筛选高亲和力抗体提供了良好的平台。
In this study, a new method based on immobilized metal affinity chromatography (IMAC) for screening phage antibody library against antigen in solution was reported.
本研究报道一种基于固定化金属亲和层析(IMAC)的噬菌体抗体库液相筛选方法。
Conclusion: Human genetic engineering antibody, which is able to react specifically to tetanus toxin, is obtained through screening phage antibody library.
结论：利用噬菌体抗体库技术筛选可得到与破伤风外毒素特异性结合的人抗破伤风类毒素基因工程抗体。
The transformed cells were infected with M13K07 helper phage to produce a phage form of library.
用辅助噬菌体m 13k07感染转化菌，以挽救出噬菌体形式的抗体库。
AIM and METHODS: The Ph. D. -7 phage display library was used to isolate peptides specific for glioma SWO-38 cell by whole cell screening.
目的和方法：用噬菌体随机7肽库对神经胶质瘤母细胞(SWO - 38)进行全细胞筛选，并通过竞争结合实验检验所筛选出的克隆的结合特异性。
These Abs were used for biopanning of a phage-displayed random15- peptide library.
并以此为筛选配基，对噬菌体表面展示的随机15肽库进行亲和筛选。
We must prove that there is really brain glioma antibody in the blood of patients, then we can establish the base for constructing humanized phage antibody library.
为了构建人源性抗脑胶质瘤抗体库，我们必须先从患者的血液内检测到抗脑胶质瘤抗体，才能为建立人源性噬菌体抗体库奠定基础。
Conclusion Human anti-keratin scFv is obtained from a large phage antibody library, which will benefit to the application of anti-keratin antibody to clinical research.
结论利用噬菌体抗体库技术获得了人源性抗角蛋白单链抗体，为临床应用研究提供了具有更广阔应用前景的人源小分子抗体。
Specific antibody was screened by 3 rounds of panning of phage antibody library with the fusion protein. The antigen binding activity and DNA sequences of positive clones were determined and analyzed.
以该融合蛋白为固相抗原，对噬菌体抗体库进行3轮淘筛，并对所获阳性克隆进行抗原结合活性测定和DNA序列测定。
Conclusion:Thiocyanate elution is a powerful and effective method to help obtain the Phabs with high affinity from phage antibody library.
结论：硫氰酸盐洗脱法用于噬菌体抗体的筛选有助于成功获得期望的高亲和力抗体。
By using combinatorial library and phage display technologies on a hapten-specific antibody we were able to demonstrate that a peripheral carrier imprint indeed exists.
利用组合库和噬菌体展示技术，我们证明半抗原特异性抗体确实存在有外围载体的某些遗迹。
Objective to find the site of the insulin binding with the insulin receptor via biopanning of disulfide constrained phage display heptapeptides library.
目的用肽库筛选技术寻找与胰岛素受体结合的胰岛素位点。
Objective: to select the peptide binding to influenza virus neuraminidase from phage display 12 peptide library.
目的：从噬菌体呈现12肽库中筛选与流感病毒神经氨酸酶特异性结合的肽。
Objective to further reconstruct the new phage display vector for more effectively displaying the foreign random peptide library.
目的进一步改造噬菌体展示载体，使之能更有效地展示各种外源随机多肽文库。
Objective To screen the mimic antigen epitopes of Schistosoma japonicum from phage display peptide library.
目的从噬菌体肽库筛选日本血吸虫抗原模拟抗原表位。
A large human phage antibody library was subjected for panning with leukocytes from healthy donors and leukemia patients to select for specific antibodies against leukocytic surface proteins.
采用正常人和白血病患者的白细胞对人源噬菌体抗体库进行淘选，以获得对两种细胞表面蛋白特异的抗体。
Objective: to establish a lung cancer phage-peptide library and to screen for biomarkers for early detection of lung cancer.
目的：构建肺癌噬菌体展示肽库，筛选出肺癌早期检测的分子标志群。
METHODS: Using CHO cells which high express human CD59 as target cells, the phage 12 peptide library was screened for 5 rounds by competitive binding test.
方法：以高表达人CD59的中国仓鼠卵巢细胞（CHO）为靶细胞，采用竞争结合试验，对噬菌体随机12肽库进行5轮亲和筛选。
The phage antibody library technology is a novel method with many potential applications, consisting of antibody library and phage surface display technology.
噬菌体抗体库技术是抗体基因文库技术和噬菌体表面展示技术相结合形成的一项新技术与方法，在生物科学领域极具潜力。
AIM: To construct phage human anti idiotypic antibody library.
目的：构建噬菌体人源抗独特型抗体库。
AIM: To obtain in situ pairing of the variable region genes of the heavy and light chains of vitiligo patients' lymphocytes, then construct and screen a human ScFv phage antibody library.
目的克隆白癜风患者淋巴细胞中原始配对的自身抗体基因，构建噬菌体抗体库并筛选针对黑素细胞膜抗原的特异性抗体。
After 4 rounds of screening with lung adenocarcinoma cell line A2 as antigen, an enriched secondary phage display library was obtained.
以肺腺癌细胞系a2为抗原进行4轮筛选，得到富集的次级噬菌体表达文库。
Objective to screen polypeptides binding to human CD137 by biopanning phage display random heptapeptide library.
目的应用噬菌体随机七肽库筛选与人CD 137特异结合的肽序列。
Objective To construct human phage antibody library and produce human monoclonal antibodies.
目的构建人噬菌体抗体组合文库，筛选人单克隆抗体。
Conclusion Error-prone PCR is an effective and simple method for affinity maturation of antibodies isolated from a phage antibody library.
结论错配pcr结合噬菌体展示技术是提高单链抗体亲和力的一种有效且简便的手段。
Objective To obtain the mimic epitope of specific and sensitive diagnostic antigen in schistosomiasis japonica from phage 12-mer peptide library.
目的从噬菌体十二肽库中筛选出特异、灵敏的日本血吸虫病诊断抗原。
Objective To obtain the mimic epitope of specific and sensitive diagnostic antigen in schistosomiasis japonica from phage 12-mer peptide library.
目的从噬菌体十二肽库中筛选出特异、灵敏的日本血吸虫病诊断抗原。
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