Methods Semi-quantitative PCR method was used in this research to estimate PERV copy-number of four mini-pigs in China.
方法采用半定量pcr方法检测了我国四种小型猪内源性逆转录病毒基因的拷贝数。
In this article, we reviewed the quantitative method and research progress of digital PCR technology in the main application fields.
本文介绍数字P CR技术的定量方法，并评述该技术在主要应用领域的研究进展。
Methods an Alkaline lysis method was used to isolate serum HBV-DNA for fluorescence quantitative PCR and compared with conventional phenol-chloroform extraction method and boiling lysis method.
方法使用碱裂解法、酚氯仿法和煮沸裂解法同时提取血清hbv - DNA，比较荧光定量pcr测定的重复性和测定值的差异。
This research established a sensitive quantitative PCR method for SIV load testing. The creteria for accuracy judgement of duplication in quantitative PCR method was introduced.
本研究建立了敏感的SIV定量PCR检测方法，并提出了判断复孔检测结果是否准确的计算方法。
Uses real-time fluorescence quota PCR the method to carry on the quantitative analysis.
采用实时荧光定量pcr的方法进行定量分析。
Using fluorescence quantitative PCR method of quantitative analysis.
采用荧光定量pcr的方法进行定量分析。
Conclusion a higher level of consistency and repeatability was found from the method of semi-quantitative PCR-RFLP compared to PCR-RFLP.
结论应用半定量pcr方法可明显提高P CR -RFLP酶切法的精确性和可重复性。
Results showed that 1. fluorescent quantitative RT-PCR was an efficient quantification method to detect the gene expression level and has a prospecting application in clinic;
结果显示，1．荧光定量RT干CR方法是一种定量检测基因表达丰度的有效方法，具有很好的临床应用前景；
Quantitative RT PCR method were used to detect the mRNA levels of multidrug resistance gene in 32 leukemia children.
应用逆转录PCR结合同位素定量分析，对32例儿童白血病患者的多源耐药基因表达水平进行了研究。
Quantitative RT PCR method were used to detect the mRNA levels of multidrug resistance gene in 32 leukemia children.
应用逆转录PCR结合同位素定量分析，对32例儿童白血病患者的多源耐药基因表达水平进行了研究。
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