Method the main influential factors were detected by PCR in serial concentration gradient and electrophoresis, then select the optimal experimental conditions.
方法对实验研究中的主要影响因素设置各系列浓度梯度，进行PCR反应后观察电泳结果选取最适条件。
Methods: Gradient PCR was employed in analysis of aldosterone synthase (CYP11B2) gene polymorphisms, and gel Imaging Technology was used to determine the optimal annealing temperature.
方法：采用梯度PCR对醛固酮合酶基因(CYP11B2)进行扩增，通过凝胶呈像技术判断CYP11 B2基因最佳退火温度。
Result: Compared with normal PCR, Gradient PCR could identify the the optimal annealing temperature of aldosterone synthase gene.
结果：与普通pcr相比，梯度PCR能够迅速地确定CYP11B2基因的最佳退火温度。
Result: Compared with normal PCR, Gradient PCR could identify the the optimal annealing temperature of aldosterone synthase gene.
结果：与普通pcr相比，梯度PCR能够迅速地确定CYP11B2基因的最佳退火温度。
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