Objective: To investigate the application of differential display-PCR (DD-PCR) in research on gene expression of Candida.
目的探讨差异显示P CR技术(DD - PCR)在念珠菌基因表达研究中的方法学要点。
Conclusions Detection of target gene expression in paraffin-embedded tissues is feasible by RT-PCR or RQ-PCR.
结论应用RT-PCR或RQ-PCR方法在石蜡包埋组织中检测目的基因的表达是可行的。
Method The expression of WT1 gene and its DNA methylation status were assayed in leukemia cell lines and normal peripheral blood mononuclear cells (PBMNC) by RT-PCR and MS-PCR.
方法采用RT P CR技术、硫化pcr结合限制性内切酶技术检测白血病细胞系及正常人外周血单个核细胞WT 1基因的表达及其启动子区DNA甲基化水平。
Myocardial ACE2 mRNA expression was respectively determined by real-time PCR (RT-PCR) in control group and diabetes group.
采用实时定量pcr (RT - PCR)检测正常及糖尿病大鼠心肌组织ace2的基因表达。
Myocardial ACE2 mRNA expression was respectively determined by real-time PCR (RT-PCR) in control group and diabetes group.
采用实时定量pcr (RT - PCR)检测正常及糖尿病大鼠心肌组织ace2的基因表达。
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