Such an accident would yield false results, especially if PCR amplification is used.
这样的偶然事件会产生错误的结果，尤其是当使用pcr扩大程序时。
A method for quickly preparing PCR amplification of dinoflagellate total DNA template was reported.
报道了一种快速制备P CR扩增甲藻细胞模板dna的方法。
Methods: PCRbased cycle DNA sequencing is a method that combines PCR amplification with DNA sequencing.
方法：PCR循环测序法是将PCR扩增与核酸序列分析相结合的一种研究方法。
Conclusions: Using PCR amplification products to prepare gene chip probe was a quickly, simple effective method.
结论：利用PCR扩增产物制备基因芯片探针是一种快速、简便、实用的方法。
Methods:The techniques including G banding, karyological analysis, PCR amplification and silver staining were used.
方法：应用染色体G分带、核型分析和PCR及银染技术。
Particularly the method of microsatellite primer-PCR amplification makes clear the relationship of genetic diversity.
尤其是微卫星引物pcr扩增明确了桃蚜寄主生物型和体色生物型的遗传分化关系。
Objective: To evaluate specificity and reliability of IS6110-based PCR amplification assays for Mycobacterium tuberculosis.
目的：评价PCR扩增is6110检测结核分枝杆菌复合群的特异性和可靠性。
The present invention is used as probe and PCR amplification detection sequence for gene chip diagnosis for candida albicans.
本发明作为基因芯片诊断的探针及PCR扩增检测序列，用于白色念珠菌的诊断。
Additionally, the inhibitors would also interference with the PCR amplification to generate false-negative detection results.
此外，抑制成分的存在也会干扰PCR扩增反应，出现假阴性检测结果。
Designed the primers of the other 15 new microsatellites, and 10 primers could obtain targeted fragments by PCR amplification.
对其余的15个微卫星设计引物，有10对引物扩增出目的片段。
Objective To study the fetal DNA in maternal plasma using multiplex PCR amplification of the short tandem repent (STR) systems.
目的采用短串联重复序列（STR）多态位点的复合扩增方法，研究孕妇血浆中胎儿DNA基因型。
The method to extract genome DNA from old skeletal specimens of cetaceans was improved and the PCR amplification reaction was optimized.
改进了从鲸类陈旧骨骼标本中进行基因组dna提取和PCR扩增的方法。
Results: Sequences were aligned , and the results showed that the products of PCR amplification were the specific gene fragments of HDV.
结果：序列分析表明，所扩增的片段均属于HDV特异基因。
The results of GUS histochemical assays and PCR amplification demonstrated that the overall transformation rate of Roselle callus was 4%.
GUS活性组织化学检测和PCR扩增鉴定的结果表明，愈伤组织的转化率为4%。
Sample preparation does not require ligation or PCR amplification, avoiding the GC-content and size biases observed in other technologies.
样本准备不需要延伸或PCR扩增，避免了在其他技术中观测到的GC含量及大小偏差。
Our method extended the utility of universal primers and provided implications for primer design and PCR amplification of some other genes.
新方法扩展了“通用引物”的适用范围，并为引物设计和一些其它基因的PCR放大提供了思路。
CONCLUSIONS HBV DNA extracted from clinical samples were directly detected using PNA biosensor and PCR amplification was successfully bypassed.
结论利用肽核酸生物传感器成功地绕过了PCR扩增而直接检测出了临床标本中的HBV基因组dna。
Result in the PCR amplification sensitivity testing revealed that 0.021 egg DNA template would be enough to amplify this specific positive band.
其PCR扩增敏感性检测结果表明，0.021个虫卵dna模板即可扩增出该特异性阳性条带。
Therefore, the paper suggests PCR amplification DNA is a highly sensitive and specific method for diagnosing tuberculous exudative pleurisy early and rapidly.
提示PCR扩增DNA技术对结核性渗出性胸膜炎是高度敏感和特异的早期、快速诊断方法。
Test results are as follows: (1) Potato PCR amplification reaction system and procedure are be fixed by optimizing PCR reaction system and selecting suitable primers.
试验过程得到如下结果：(1)对马铃薯PCR反应体系进行了优化，筛选出合适的引物，确定马铃薯P CR反应体系和扩增程序。
Conclusion RNA can be extracted from the OCT-embedded long-stored tissues treated in 25% sucrose in PBS. And all the extracted RNA can be used to RT-PCR amplification.
结论从25%蔗糖磷酸缓冲液固定oct包埋的冰冻时间较长的组织标本中可提取组织rna，并用于进行RT - PCR扩增。
It relies upon the selective deamination of unmethylated cytosine to uracil after treatment with sodium bisulphite, usually followed by PCR amplification of the chosen target region.
它依赖于用亚硫酸氢钠处理后，非甲基化的 胞嘧啶选择性的去氨基为 尿嘧啶，通常接着所选的靶向区域的PCR扩增。
Objective To construct the recombinant plasmid that highly expressed human zona pellucida 3. Methods The techniques of PCR amplification, T-A vector ligation, and sub-clone were used.
目的：构建高效表达人透明带蛋白3的真核重组表达载体。方法：利用PCR、T- A载体克隆和亚克隆等技术。
Based on the GDNF gene sequence of human and chick, we cloned partial DNA sequences encoding mature protein of GDNF from genomic DNA of giant panda and crested ibis through PCR amplification.
本项研究经pcr引物设计后，以大熊猫和朱鹮的基因组d NA为模板，克隆了大熊猫和朱鹮的GDNF成熟蛋白基因。
Methods The telomerase activity was detected by TRAP( telomeric repeat amplification protocol) -ELISA which based upon PCR amplification of the initial telomerase product and detected by ELISA.
方法采用端粒重复序列扩增文件-酶标法（TRAP-ELISA）测定尖锐湿疣皮损中端粒酶的活性，并与正常皮肤和恶性肿瘤对照比较。
The reactions are typically carried out on a regulated heating block, or PCR machine, and consist of 30-35 cycles of repeated amplification of all the DNA present.
这一反应通常是在一可调控的温箱中或PCR仪中进行，对所有的DNA进行30到35个循环扩增。
The reactions are typically carried out on a regulated heating block, or PCR machine, and consist of 30-35 cycles of repeated amplification of all the DNA present.
这一反应通常是在一可调控的温箱中或PCR仪中进行，对所有的DNA进行30到35个循环扩增。
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