METHOD Whole-cell patch clamp technique was used.
方法采用全细胞膜片钳技术。
METHODS Using cell-attached patch clamp technique.
方法采用细胞贴附式膜片钳技术。
Methods Whole-cell patch clamp technique was used.
方法采用全细胞膜片钳制技术。
METHODS Whole cell recording by patch clamp technique.
方法膜片钳全细胞记录。
METHODS Patch clamp technique with whole cell configuration.
方法膜片钳制技术全细胞记录模式。
METHODS: Cell-attached configuration of patch clamp technique.
方法：细胞贴附式膜片钳技术。
Abstract: the study of lens cells by patch clamp technique began in the early 90 "s."
文摘：应用膜片钳技术对晶体细胞的研究始于90年代初。
Methods: Whole-cell patch clamp technique was used to determine sodium channel cur rent.
方法：采用全细胞膜片钳技术，记录钠通道电流。
Whole cell patch clamp technique was used to record the action potential and ion currents.
以全细胞膜片钳记录单细胞跨膜动作电位和离子电流。
Methods The data was recorded by adopting inside out configuration of patch clamp technique.
方法采用膜片钳技术内面向外式。
The widely using of patch clamp technique has greatly promoted our knowledge of ion channels.
膜片钳技术的广泛应用极大推动了人们对离子通道的认识。
Methods The whole cell patch clamp technique was used in isolated ventricular cells of guinea pigs.
方法：用膜片钳实验技术记录胶原酶急性分离的豚鼠心室肌细胞钾通道电流。
Single channel currents were recorded by using the patch clamp technique in inside-out configuration.
急性分离单个的豚鼠气管平滑肌细胞，以膜片钳技术的内面向外式记录单通道电流。
METHODS: Patch clamp technique (whole cell recording) was used to record L-Ca2 + current in single cardiac myocyte.
方法：用全细胞膜片箝记录心室肌细胞的L -钙电流。
CONCLUSION: the patch clamp technique makes it possible to observe the action sites of nicotine receptor antagonists.
结论：膜片钳技术使探讨烟碱受体拮抗药的作用位点成为可能。
Methods: By using patch clamp technique, measure voltage gated calcium currents in undifferentiated SH SY5Y cell line.
方法：通过膜片钳手段，测量未分化的SHSY5Y细胞钙离子通道电流；
The first step of recording calcium channel current of cardiomyocytes with patch clamp technique is dissociation of myocytes.
用膜片箝技术记录心肌细胞钙通道电流，首先必须获得单个心肌细胞。
Meynert nucleus was obtained and dissociated to observe their morphology and record electric activity by patch clamp technique.
作形态学观察，通过单细胞膜片钳技术记录电活动。
Methods: The isolated neurons of dorsal root ganglion (DRG) were studied using the whole cell patch clamp technique in current clamp mode.
方法：应用全细胞膜片箝电流箝制技术，对急性分离的大鼠DRG细胞进行电流箝制。
The electric physiology feature of L-type calcium channels in the vascular smooth muscle cells was analyzed by patch clamp technique (PCT).
用全细胞模式膜片钳技术记录分析血管平滑肌细胞L型电压依赖性钙通道的特性。
The signal of single ion channel is stochastic ionic currents on the order of 1pA, which can be recorded by means of patch clamp technique.
细胞膜离子单通道信号是皮安级的随机电流，膜片钳技术可以记录这些信号。
Methods Enzyme digestion was used to isolate single ventricular myocyte and whole cell patch clamp technique was used to record potassium current.
膜片钳技术是一种以记录离子通道的离子电流来反映细胞膜上单一的或多个的离子通道分子活动的技术。
MethodsAfter rat hippocampal or cerebral cortical neurons were isolated by pronase e, the IA currents were recorded by whole-cell patch clamp technique.
方法用急性酶解法获得单个大鼠心室肌细胞，标准的全细胞膜片钳技术记录钠通道电流。
Patch clamp technique is a kind of technique which reflecting membrane molecular activity of one or more ion channel by record ion current of ion channel.
膜片钳技术是一种以记录离子通道的离子电流来反映细胞膜上单一的或多个的离子通道分子活动的技术。
This article reviewed lately research status of Chinese herbal effect on calcium channels of myocardial cell by patch clamp technique and look ahead the future research.
本文综述了近年来利用膜片钳技术研究中药对心肌细胞膜钙通道作用的现状，并对未来研究进行展望。
The characterizations of voltage-activated potassium channels were studied on newborn bovine retinal neurons in long-term cultures with whole-cell patch clamp technique.
目的探讨体外培养不同时期新生小牛视网膜神经元电压门控钾离子通道的特性。
Whole-cell patch clamp technique was used to study the electrophysiological characteristics of nociceptive trigeminal ganglion (TG) neurons labeled by nuclear yellow (NY).
实验利用全细胞膜片钳技术研究了荧光染料核黄（NY）标记的支配牙髓的痛感觉神经元的电生理特征。
Methods Cell-attached mode of patch clamp technique were employed to record the single channel current of NMDA receptor with the primary-cultured hippocampal neurons from rats as samples.
以原代培养的大鼠海马神经元为标本，运用膜片钳技术的细胞吸附式方法记录NMDA通道的单通道电流。
METHODS The patch clamp whole cell recording technique was used.
方法采用膜片钳全细胞式记录技术。
Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.
方法传代得到单个HERG-HEK细胞，应用全细胞膜片钳技术记录HERG-HEK细胞上的HERG钾电流和动力学曲线（激活、失活、复活和去活化）。
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