Methods We used the improved enzymatic dissociation method and the patch clamp whole cell recording.
方法采用改进的酶解分离法分离单个细胞并经膜片钳全细胞记录法进行鉴定。
Conclusion a simple and fast method for acutely isolating rats cortex neurons suitable for patch clamp is successfully found.
结论成功建立了一种简单快速的适用于膜片钳技术的大鼠皮层神经元急性分离改良方法。
A combination of mechanical and enzymatic isolation method is described for acutely isolating rat hippocampal neurons suitable for patch clamp recording.
结合酶消化和机械分离的方法，探讨一种适用于膜片箝记录的大白鼠海马神经元的急性分离技术。
METHOD Whole-cell patch clamp technique was used.
方法采用全细胞膜片钳技术。
The automatic patch-clamp system had been analyzed and modeled by using the method of UML in RUP.
通过基于RUP的UML系统建模方法对全自动膜片钳软件系统进行了分析和建模。
Method: the whole cell patch clamp recording technique was used in this study. Results: blood resin suppressed amplitude of the sodium channel currents in a dose dependent way.
方法：采用全细胞膜片钳记录法，在新鲜分离的大鼠背根神经节细胞上观察血竭对钠通道电流幅值的影响。
Objective: to devise a method to isolate calcium-tolerant cardiomyocytes from SD rats for the experiment of patch clamp, and record the currents of L-type calcium channels.
目的：建立大鼠心肌细胞分离方法，用于膜片钳实验，并记录L型钙离子通道电流。
In this paper we present an easily available method of intracellular dialysis via a microcatheter inserted into glass pipette during patch clamp experiment.
本文介绍了一种膜片钳微电极内插管进行胞内透析的方法。
The visual patch-clamp method can accurately assess synaptic transmission by measuring postsynaptic currents of the dorsal horn neurons.
可视膜片钳方法可以准确测量脊髓背角神经元的突触后电流从而研究初级传入突触的传递过程。
Method The experiment was carried out in freshly isolated bullfrog using a whole-cell patch-clamp technique.
方法实验采用的方法是全细胞膜片钳技术，实验材料为急性分离的牛蛙背根神经节神经元。
An stable method for human myocytes ion-channel patch-clamp recording were established.
应用此系统我们建立了稳定的人心肌细胞离子通道膜片钳记录方法。
Method: With conventional Patch Clamp whole-cell recording technique.
方法：采用常规膜片钳全细胞记录技术。
Method: With conventional Patch Clamp whole-cell recording technique.
方法：采用常规膜片钳全细胞记录技术。
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