The tumor spheres were passaged and expanded.
将培养出肿瘤细胞克隆球给予传代扩增；
The cells were passaged by mechanical methods, frozen and thawed by general procedure.
采用机械方法传代，常规方法冻存和复苏。
Methods: The fetal RPE cells were cultured with trypsin enzyme digesting technique and passaged in vitro.
方法：用胰酶消化法获取人胎儿视网膜色素上皮细胞进行原代及传代培养。
Methods: The rabbit RPE cells were cultured with trypsin enzyme-digesting technique and passaged in vitro.
方法：用胰酶消化法培养兔rpe细胞并传代。
Oral squamous cells carcinoma cells lines were cultured and passaged, the conditional medium of tumor cells were prepared.
传代培养口腔鳞癌细胞株，制备肿瘤细胞条件培养液；
In contrast, EGF-responsive neural stem cells proliferated more rapidly, migrated and differentiated less, and were passaged more easily.
相反，EGF反应性神经干细胞快速增殖，易于传代，较少迁移和分化。
In control group es cell -like colonies were harvested from 9 blastocysts, in which 5 es cell-like colonies could be passaged successfully.
对照组卵丘细胞重构囊胚中9个囊胚中分离出es细胞样集落，5个ES细胞样集落可稳定传代。
Results Human amniotic epithelial cells were successfully cultured and passaged in vitro, and could be passaged successively for 8-10 times.
结果人羊膜上皮细胞可以在体外成功的培养传代，体外可连续传8 ~10代。
Methods Amnion cells from human were cultured with tissue piece method, passaged by trypsin digestion and identified with immunocytochemistry.
方法采用组织块培养法培养羊膜细胞，酶消化法对细胞进行传代，应用免疫细胞化学的方法对细胞进行鉴定。
Conclusion Human amniotic epithelial cells can be successfully primary cultured and passaged in vitro, and the cells can proliferate for a certain period.
结论人羊膜上皮细胞在体外可成功进行原代和传代培养，体外培养的人羊膜上皮细胞在一定时间内可维持增殖能力。
Methods Chondrocytes isolated from articular cartilage and meniscus of rabbits aged 3 weeks were respectively passaged in monolayer and cultured in centrifuge tube.
方法分别自3周龄大耳白兔关节软骨和半月板分离软骨细胞，行单层传代培养和离心管培养。
METHODS: Primary human periodontal ligament cells were obtained and passaged. Then the cells were stretched by dynamic mechanical strain unit with 1%, 10% and 20% strain at different time.
方法：通过原代和传代培养，获得性状稳定的人牙周膜细胞，使用动态机械应变细胞加载仪对细胞进行1%、10%和20%动态牵张应变加载。
METHODS: Primary human periodontal ligament cells were obtained and passaged. Then the cells were stretched by dynamic mechanical strain unit with 1%, 10% and 20% strain at different time.
方法：通过原代和传代培养，获得性状稳定的人牙周膜细胞，使用动态机械应变细胞加载仪对细胞进行1%、10%和20%动态牵张应变加载。
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