Similar results were obtained in paraffin embedded and frozen sections.
石蜡和冰冻组织标本结果一致。
Methods In situ hybridization was used determine the expression in routinely paraffin embedded sections.
方法石蜡包块切片组织原位杂交染色法。
Conclusion: Appropriate DNA extract methods for formalin fixed and paraffin embedded tissues may benifit PCR.
结论:合适的福尔·马林固定石蜡包埋组织dna提取方法可应用PCR进行DNA水平的相应的研究。
Method Immunohistochemical technique for MAGE-3 antigen was performed in paraffin embedded sections of 101 CRC specimens.
方法应用免疫组化法对101例大肠癌组织石蜡标本进行MAGE-3抗原表达测定。
Method Using LSAB immunohistochemical technique to detect the expression of MAGE 3 antigen in formalin fixed, paraffin embedded sections.
用免疫组化LSAB的方法对石蜡包埋的组织切片进行MAGE抗原的检测。
Objective To study the sensitivity and specificity of single tube nested polymerase chain reaction (SN PCR) technique in detecting Mycobacterium tuberculosis DNA in paraffin embedded tissues.
目的探讨单管巢式聚合酶链反应(SNPCR)检测石蜡包埋组织结核分支杆菌dna的特异性和敏感性。
Owing to the DNA degradation induced by formalin and the obstruction of paraffin to DNA extraction, it is difficult to recover high-quality DNA from Formalin fixed and paraffin embedded tissue(FFPET).
由于甲醛介导的DNA损伤和石蜡对DNA提取的阻碍作用,使得常规DNA提取方法很难从福尔马林固定石蜡包埋组织(FFPET)中获取高质量的DNA。
Representative pieces of periprosthetic tissue were embedded in paraffin blocks for routine sectioning and staining with hematoxylin and eosin.
假体周围组织的典型地区分别嵌入常规切片和苏木精和伊红染色石蜡块。
The tissues were fixed in 10% formalin and embedded in paraffin. No patients had received preoperative chemotherapy , radiation therapy or other biological therapy.
全部组织均经10%福尔马林固定,石蜡包埋,术前均未行放疗,化疗或者其他生物治疗。
However, the major obstacle to use technique as a routing is its high false negative rates and inconstant results, especially in paraffin-embedded tissues.
然而,假阴性率高、结果不稳定等因素严重阻碍着该技术的广泛应用,在石蜡组织中尤其如此。
Methods 32 human salivary ACC paraffin-embedded tissue samples were divided into two groups according to the pathological presence of perineural invasion.
方法根据嗜神经侵袭的病理现象,将32例人涎腺腺样囊性癌(ACC)石蜡组织划分为未见神经侵袭组和存在神经侵袭组。
Conclusions Detection of target gene expression in paraffin-embedded tissues is feasible by RT-PCR or RQ-PCR.
结论应用RT-PCR或RQ-PCR方法在石蜡包埋组织中检测目的基因的表达是可行的。
By the optimization of experimental conditions, FISH can be successfully performed on paraffin-embedded tissue.
通过优化实验条件,可以在石蜡包埋组织上成功地进行FISH实验。
Conclusions The way to extract RNA from paraffin-embedded tissue is stable and feasible, and it is a good method for disease-associated gene detection.
结论石蜡包埋组织中提取RNA方法稳定,可为疾病相关基因检测提供有效手段,在科研和临床方面有广阔的应用前景。
The DNA content and cell cycle in paraffin-embedded sections collected from 5 cases of congenital mesoblastic nephroma. 96 cases of nephroblastoma and 27 normal kidneys were studied by flow cytometry.
应用流式细胞术对5例先天性中胚层肾瘤、96例肾母细胞瘤和27例正常肾石蜡包埋组织进行了DNA含量和增殖活性分析。
Methods: Immunohistochemistry was used to detect the expression of CENP- F in 64 cases of brain glioma and 10 cases of normal brain tissues by paraffin- embedded.
方法:采用免疫组化方法检测64例脑胶质瘤和10例正常脑组织石蜡包埋标本中CENP-F的表达。
All specimen were fixed by formalin and embedded by paraffin.
所有标本经福尔·马林固定,石蜡包埋。
Methods: Fresh health premolars were collected, divided into some groups according to age, embedded in paraffin and cut into slices.
方法:收集健康新鲜的前磨牙,按年龄分组,石蜡包埋切片,免疫组织化学与图像定量分析。
Cancer tissues were embedded in paraffin and stored for 13.4 years in average.
样品为石蜡包埋的肺肿瘤组织块,平均保存13.4年。
All of these specimens were fixed in 10% neutral formalin and then embedded in paraffin.
取材后用10%中性福尔·马林固定,常规石蜡包埋。
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using 53bp1 Antibody in the presence of control peptide (left) or antigen-specific peptide (right).
免疫组织化学方法检测石蜡包埋的人结肠癌组织,使用的抗体为53bp1。左图是对照组,右图是抗原特异性肽段组。
Objective: To study the EB virus DNA distribution in paraffin-embedded tissue, peripheral blood leukocyte and serum in nasopharyngeal carcinoma patients (NPC).
目的:探讨鼻咽癌(NPC)组织及患者外周血白细胞和血清中eb病毒DNA的分布及其与NPC的关系。
Results 100 % of frozen tissues and 24.1% of paraffin-embedded tissues could get either methylated production or unmethylated production.
结果100%的新鲜组织和24.1%的石蜡包埋组织至少可以得到甲基化和非甲基化产物中的一种产物。
Methods: Using sp immunohistochemical method, the PCNA indices and positive rate of P53 were examined in 46 paraffin-embedded specimens of tongue cancer diagnosed by pathology.
方法:应用免疫组化SP法,观察46例病理确诊的舌癌石蜡标本中pcna指数及P53阳性率的表达。
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using FAK Antibody in thre presence of control peptide (left) or antigen-specific peptide (right).
在对照肽(左)或抗原特异性多肽(右)存在的条件下使用FAk抗体对石蜡包埋的人肺癌组织进行免疫组化分析。
All specimens required to formalin-fixed, paraffin-embedded.
所有标本按要求以福尔·马林固定,石蜡包埋。
Objective To examine the alteration and significance of the DPC4 gene in paraffin-embedded tissues of pancreatic carcinomas.
目的研究石蜡包埋胰腺癌组织中DPC4基因的改变。
The prognostic significance of RASSF1A methylation was validated in 93 formalin fixed paraffin-embedded (FFPE) tissues obtained from patients with operable breast cancer.
从患可手术的乳腺癌患者中获得93例福尔·马林固定石蜡包埋(FFPE)的组织,来验证RASSF1A甲基化的预后重要性。
PCR technique was used to detect the IS6110 specific insertion sequence DNA of Mycobacterium tuberculosis complex in the 120 paraffin-embedded tissues.
提取120例拟诊为结核病的石蜡包埋组织样本dna,经酚-氯仿-异戊醇纯化后用PCR技术扩增结核分枝杆菌特异序列is6110片段。
PCR technique was used to detect the IS6110 specific insertion sequence DNA of Mycobacterium tuberculosis complex in the 120 paraffin-embedded tissues.
提取120例拟诊为结核病的石蜡包埋组织样本dna,经酚-氯仿-异戊醇纯化后用PCR技术扩增结核分枝杆菌特异序列is6110片段。
应用推荐