Objective:To obtain the mimotope of avian infectious bronchitis virus by phage display peptide library.

目的：利用噬菌体展示肽库技术筛选鸡传染性支气管炎病毒的模拟抗原表位。

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Methods (1) The coding sequence of MG7 mimotope (GC23) was included in the reverse primer, and by PCR incorporated with HSP70 gene at the 3 terminus.

方法1。根据HSP70全长基因序列设计针对其编码区的引物，在下游引物的宁端加人MG7模拟表位的核昔酸序列。

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A competitive ELISA was set up with a mimotope peptide conjugated to BSA carrier, and the new method was compared with conventional ELISA method and HPLC method.

将化学合成的模拟表位肽与载体BSA偶联后，以之建立无毒素的竞争酶联免疫检测方法，并与常规酶联免疫吸附检测方法及高效液相色谱比较。

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A competitive ELISA was set up with a mimotope peptide conjugated to BSA carrier, and the new method was compared with conventional ELISA method and HPLC method.

将化学合成的模拟表位肽与载体BSA偶联后，以之建立无毒素的竞争酶联免疫检测方法，并与常规酶联免疫吸附检测方法及高效液相色谱比较。

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