METHODS: Mouse lymphoma EL4 cells were inoculated subcutaneously into wild type C57BL/6 mice (immune-competent mice).
方法：给正常野生型C57BL/6小鼠皮下接种小鼠淋巴瘤EL4细胞，建立荷EL4肿瘤的小鼠模型。
Methods To introduce DNA encoding the wild- type and mutant channel into immortalized tyrosine hydroxylase- positive CNS- derived neurons, CAD cells.
方法给一类酪氨酸脱氢酶阳性的神经细胞（一种中枢神经源细胞），又称CAD细胞，引入由DNA编码的野生型和突变型离子通道。
In current, different kinds of methods were widely used in the animal model, such as the wild type, congenital defect and gene modification.
目前野生型、先天缺陷型、基因修饰等动物模型被广泛应用在这一研究领域。
METHODS: The regulating region of the human GCLC gene was cloned by PCR method to construct the wild type plasmid, which expressed luciferase reporting gene.
方法：通过PCR的方法克隆GCLC基因的部分转录调控区基因，构建表达虫荧光素酶报告基因的质粒；
METHODS: The regulating region of the human GCLC gene was cloned by PCR method to construct the wild type plasmid, which expressed luciferase reporting gene.
方法：通过PCR的方法克隆GCLC基因的部分转录调控区基因，构建表达虫荧光素酶报告基因的质粒；
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