Polymerase chain reaction-based (PCR-based) and enzyme-linked immunoabsorbent assay (ELISA) methods are now applied in the detection of major food-borne pathogens.
依据聚合酶链反应(PCR)和酶联免疫吸附检测(ELISA)的方法目前被应用于主要食源性致病菌的检测。
Methods: IL-8 levels of serum and urine were determined by Sandwich-ELISA within 47 NS active period and 8 weeks after treating with steroid impact, 38 healthy volunteers were used as control.
方法：采用ELISA夹心法测定47例肾病综合征患者在疾病活动期和激素冲击治疗8周后血清及晨尿IL-8的水平，以38例健康体检者作对照。
Methods The plasma levels of CD146 were detected by enzyme linked immunosorbent assay (ELISA) in 11 chronic glomerulonephritis patients and 10 normal controls.
方法用ELISA方法(酶联免疫吸附实验)检测11例慢性肾炎患者与10例正常人外周血cd146的表达。
Methods the neural stem cells were isolated, identified and amplified from the cortex of aborted human embryo with 10 to 14-week gestational age, then telomerase activity was detected by PCR-ELISA.
方法分离、鉴定并扩增取自10 ~ 14周药物流产胎儿大脑皮质的神经干细胞，应用pcr - ELISA法检测神经干细胞端粒酶活性。
Methods: Using the method of double antibody sandwich ELISA detected the MBP antigen and anti-MBP antibody.
方法：MBP抗原和抗体的测定均采用双抗体夹心ELISA法测定。
Methods the serum of 123 cases of patients were detected with ECLIA and ELISA respectively, and the repeatability tests were carried on, then.
方法将123例患者的血清分别用化学发光法和酶联免疫法检测，并做重复性实验。
It may be that the antigen structure was effected by the different ELISA methods, so that the capability of the binding of antigen to antibody was changed.
这可能由于ELISA方法对抗原结构的影响而导致抗原抗体结合的不同。
Methods Affinity constant of the specific antibodies was determined with ELISA and capillary electrophoresis.
方法采用ELISA和毛细管电泳测定抗体抗原的亲和常数。
Methods The mice were immunized by different doses of cholera vaccine. Titer of antiserum against cholera strain and toxin were tested by indirect enzyme-linked immunosorbent assay (ELISA).
方法以不同剂量霍乱疫苗免疫小鼠，用间接elisa方法检测小鼠全血的抗菌、抗毒抗体滴度，小鼠毒菌攻击法计算保护率。
Methods: Electron microscopy, reticulum fiber stain and ELISA test were used to observe the pathologic pro - cess of pulmonary fibrosis and to determine the change of collagen content.
方法：应用电子显微镜，网状纤维染色和ELISA方法检测大鼠肺纤维化的病理过程和胶原含量的改变。
Methods The serum level of MMP-9 in 40 patients with IPF and 30 healthy subjects was measured by ELISA.
方法采用酶联免疫吸附法对40例特发性肺纤维化患者和30例健康人血清基质金属蛋白酶- 9进行测定。
Methods: Mixed bacterium was used for making chronic inflammatory rats model, the pathology of oviduct was observed and the content of IL-2 and IL-6 in oviduct was checked by ELISA method.
方法：用混合菌接种法制作大鼠慢性盆腔炎动物模型，观察输卵管的病理变化，并以ELISA法检测其中IL - 2、IL - 6的含量。
Methods The telomerase activity was detected by TRAP( telomeric repeat amplification protocol) -ELISA which based upon PCR amplification of the initial telomerase product and detected by ELISA.
方法采用端粒重复序列扩增文件-酶标法（TRAP-ELISA）测定尖锐湿疣皮损中端粒酶的活性，并与正常皮肤和恶性肿瘤对照比较。
METHODS: The whole blood concentrations of tacrolimus were measured by ELISA. The levels of tacrolimus in 1190 samples from 138 liver transplant recipients were compared and studied.
方法用ELISA法测定他克莫司全血浓度，对138例患者的1190例次监测结果进行比较分析。
Methods the SARS antibody in serum of vary population were detected by the method of ELISA.
方法：用酶联免疫吸附试验(ELISA)对各类人群的血清进行SARS病毒抗体的检测。
Methods: Specific antibodies were detected with enzyme linked immunosorbent assay (ELISA) using coated lung tissue as antigen and serum of SARS patients as antibodies.
方法：制备SARS患者肺组织包被抗原，采用酶联免疫吸附试验(EL IS A)检测SARS患者血清中特异性抗体。
Methods: Using ELISA method to determine the BNP levels of the heart failure group and the control group by common of 38 cases, then to made comparison with both groups.
方法：采用ELISA法测定心衰组和非心衰组共38例老年患者的血浆脑钠素水平并进行比较。
Methods Enzyme-linked immunosorbent assay (ELISA) was adopted to determine the antigen of Norwalk-like viruses.
方法应用酶联免疫吸附实验方法(ELISA)进行诺瓦克样病毒抗原检测。
The results lay the foundation for the further development of ELISA methods by using this monoclonal antibody.
这一结果为用自制单抗进一步开发酶联免疫方法检测奠定了基础。
Methods ELISA was adopted to determine the NSE contents in the serums from 49 child cases of tuberculous meningitis, and 15 healthy children (control group).
方法采用酶联免疫法测定49例结核性脑膜炎患儿和15例对照组血清中nse含量。
Methods: Plasma level of soluble CD105 was detected by ELISA in 50 patients with colorectal cancer and 31 normal subjects.
方法：采用酶联免疫吸附试验法（ELISA）对50例大肠癌患者以及31例正常人CD105浓度进行了检测。
Methods ANCA fluorescent patterns and its target antigens in sera of 205 patients with renal diseases were determined by indirect immunofluorescence (IIF) and ELISA.
方法应用间接免疫荧光(IIF)和ELISA方法检测了205例肾脏疾病患者血清中的ANCA及其靶抗原。
Methods Serum levels of endostatin were measured by an enzyme-linked immunosorbent assay (ELISA) in 44 patients with lung cancer, 20 patients with benign lung diseases and 20 normal persons.
方法应用酶联免疫吸附试验(ELISA法)，测定44例原发性肺癌、20例肺部良性疾病和20例正常人血清内皮抑素含量。
Methods ELISA was used to determine the level of IL-8 in serum in 30 newborns with severe infection, 28 newborns with mild infection and 28 healthy newborns, respectively.
方法采用酶联免疫吸附法检测30例重症感染新生儿、28例轻症感染新生儿及28例正常健康新生儿血清il - 8水平。
Methods The allergen of inhalation and edibility in 311 cases with allergic dermatitis and 30 healthy persons were detected by enzyme-linked immunosorbent assay (ELISA).
方法采用酶联免疫吸附试验(ELISA)对311例过敏性皮肤病患者和30名健康对照者血清中吸入性及食入性过敏原进行检测。
Methods: The serum level of P-selectin in 92 cases of traumatic patients was determined with ELISA.
方法：采用酶联免疫法(ELISA)测定92例创伤患者血清中P -选择素水平。
Methods The serum samples from 310 cases of viral hepatitis were tested by Fluorescence quantitative PCR assay (FQ PCR), and also by ELISA as contrast.
方法运用荧光定量pcr (FQ - pcr)和el IS A两种方法同时检测了310份肝炎患者血清，并对结果进行了对比分析。
Methods The levels of IL-4, IL-5 and ECP in the serum and induced sputum were measured with enzyme-linked immunosorbent assay (ELISA) method before and after two different treatments.
方法采用酶联免疫法检测糖皮质激素不同给药方式治疗前后外周血清及诱导痰液中IL-4、IL-5和ECP水平。
Methods An enzyme-linked immunosorbent assay(ELISA)was utilized to detect the levels of IL-18, VEGF in serum of 75 patients with HSP.
方法采用固相酶联免疫分析法检测75例过敏性紫癜患者血清IL-18、VEGF水平。
Methods Serum level of DHEAS was measured by magnetic separated ELISA in 115 patients with diabetes and 295 patients with coronary heart disease, and compared with the control.
方法采用磁分离酶联免疫法测定了115例糖尿病患者和295例冠心病患者的血清dheas水平。
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