Method 4 kinds of block buffer, the different concentration of anti-MIF and biotin-anti-MIF, the different dilution of streptavidin-HRP are used for titration of ELISA test.
方法采用方阵滴定法，比较4种封闭液、不同稀释度的捕获抗体、检测抗体和辣根过氧化酶标记链菌素对检测结果的影响，确定ELISA法的最佳实验条件。
Method 4 cases of melanoma with metastasis or recurrence after operations were vaccinated with large dose of BCG live vaccine. The long term efficacy was observed and summarized.
方法对4例恶性黑色素细胞瘤转移或术后复发患者接种大剂量卡介苗活菌的长期疗效进行观察和总结。
Thus, detergent-based cellular fractionation separates cellular components with greater ease and speed compared to a more laborious density centrifugation method (4).
因此，与费劲的密度梯度离心方法相比，基于洗涤剂的细胞法分馏能简单快速地分离细胞组分（4）。
Method: 4 cases of rheumatic mitral stenosis were treated by PBMV with single balloon catheter.
方法：采用国产单球囊导管对4例风湿性心脏病二尖瓣狭窄患者进行PBMV。
Method: 4 cases of rheumatic mitral stenosis were treated by PBMV with single balloon catheter.
方法：采用国产单球囊导管对4例风湿性心脏病二尖瓣狭窄患者进行PBMV。
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